Evaluation of fish biodiversity in estuaries using environmental DNA metabarcoding

Discussion Distribution of detected species The 11 species detected in all five rivers are common in Japan, and some of them (e.g., com- mon carp and flathead grey mullet) have a worldwide distribution [42, 43]. Some, such as Fig 3. Human effects. Nutrients (mean ± SD) including (a) total nitrogen [mg/L] and (b) total phosphorus [mg/L] of the five rivers based on 2016 data obtained from the Ministry of the Environment, Japan (https://water-repo.env.go.jp/ water-repo/). (c) Revetment rate [%] of the five rivers calculated using Google Earth Pro (2018 Google Image Landsat/ Copernicus, US Dept of State Geographer Data SIO, NOAA, U. S. Navy, NGA, GEBCO). Bars show standard deviations. https://doi.org/10.1371/journal.pone.0231127.g003 PLOS ONE Biodiversity of five estuaries PLOS ONE | https://doi.org/10.1371/journal.pone.0231127 October 6, 2020 9 / 15