Geology and climate influence rhizobiome composition of the phenotypically diverse tropical tree Tabebuia heterophylla

high altitude Bosque Estatal de Maricao 18˚ 09’ 33.4’’ N 66˚ 59’ 55.5’’ W; and Site 4 (S4) was in Cabo Rojo, low altitude (near sea level), 18˚ 07’ 51.1" N 67˚ 09’ 34.4" W. Limestone/karst areas (in this work, karst) were located in Bosque Estatal de Gua´nica (low altitude karst) 17˚58018@N 66˚54029@W and in high altitude area, Arecibo (S6), 18˚ 22’ 49.1’’ N 66˚ 41’ 09.6’’ W. Thus, each site represents a unique combination of parent material and climate (Table 1). A map rep- resenting the collection sites, was prepared using the SRTM–Shuttle Radar Topography Mis- sion Digital Elevation Data (90 m). At each site, three T. heterophylla trees (each tree represents a sampling unit) were chosen based on similar sizes, to sample the rhizosphere and nearby bulk soil. Once the tree was identified, the soil was dug nearby to identify the main root Fig 1. Map of the Island of Puerto Rico with the locations, parental material, temperature, humidity and elevation, and leaf morphology respectively, of each site. GPS coordinates are in Table 1. https://doi.org/10.1371/journal.pone.0231083.g001 PLOS ONE Rhizobiota of the tropical tree Tabebuia heterophylla PLOS ONE | https://doi.org/10.1371/journal.pone.0231083 April 7, 2020 3 / 16