Geology and climate influence rhizobiome composition of the phenotypically diverse tropical tree Tabebuia heterophylla

Supporting information S1 Table. Statistical alpha diversity comparisons between rhizosphere and soil communi- ties in each geologic and climatic site. (XLSX) S1 Fig. Dotplot of alpha diversity of combined bulk soil and rhizosphere samples, accord- ing to geology and precipitation. (PDF) S2 Fig. Rarefaction curves of bacterial diversity per tree according to geology and eleva- tion, comparing rhizosphere and bulk soil samples. (PDF) S3 Fig. PCoA plots depicting phenotypes. Panel A shows microbiomes according to leaflet number; panel B compared leaflet shape, panel C shows apex morphology, panel D shows peti- ole length, panel E shows a range of tree height and Panel F displays microbial communities based on mean annual temperature. (PDF) S4 Fig. Discriminant phyla-level analyses according to mean annual temperature (x-axis), with LEfSe (Linear discriminant analysis effect size) algorithm. The algorithm employs non- parametric Kruskal-Wallis rank sum test to detect phyla with significant differential abundance for a given category (mean annual temperature). (PDF) S5 Fig. Discriminant genus changing in abundance according to geology, using LEfSe. (PDF) S6 Fig. Discriminant genus-level analyses according to geology and microhabitat/sample type. (PDF) Acknowledgments We acknowledge the participation of Alexandra Mele´ndez, Stephany Pe´rez, Joel Va´zquez in the field sample collection. Author Contributions Conceptualization: Filipa Godoy-Vitorino. Data curation: Filipa Godoy-Vitorino. Formal analysis: Yakshi Ortiz, Carla Restrepo, Brayan Vilanova-Cuevas, Filipa Godoy- Vitorino.

Funding acquisition: Susannah G. Tringe, Filipa Godoy-Vitorino. Investigation: Susannah G. Tringe, Filipa Godoy-Vitorino. Methodology: Eugenio Santiago-Valentin, Susannah G. Tringe, Filipa Godoy-Vitorino. Project administration: Susannah G. Tringe, Filipa Godoy-Vitorino. Resources: Eugenio Santiago-Valentin, Filipa Godoy-Vitorino. Software: Filipa Godoy-Vitorino. PLOS ONE Rhizobiota of the tropical tree Tabebuia heterophylla PLOS ONE | https://doi.org/10.1371/journal.pone.0231083 April 7, 2020 13 / 16