Insights into the representativeness of biodiversity assessment in large reservoir through eDNA metabarcoding

2020–2021, including Roeboides descalvadensis, Hoplias malabaricus, Crenicichla semifasciata, Cichla kelberi, C. piquiti, Satanoperca pappaterra, Serrasalmus maculatus, Oreochromis niloti- cus, O. aureus and Plagioscion squamosissimus, for instance. Only 19 species (~14%) had GBIF records for the TI reservoir. Seventy-seven fish species were identified through eDNA when considering all locations from both sampling and station samples (Fig 3). These species are distributed among nine Fig 3. Ichthyofauna distribution and frequency across the Três Irmãos (TI) reservoir. Heatmap illustrating the distribution of fish Molecular Operational Taxonomic Units (MOTUs) identified by eDNA across sampling stations in the Três Irmãos (TI) reservoir. The TI reservoir is shown at the top with all sampled stations C1-C4 and C6-C15. Stations abbreviations as in S1 Table. Hydroelectric power plants Três Irmãos (TI) and Nova Avanhandava (NA) are shown at the beginning and at the end of the reservoir. The yellow to red color spectrum represents the log10 transformation of the average frequency. Solid black lines on the left delineate the observed orders. https://doi.org/10.1371/journal.pone.0314210.g003 PLOS ONE eDNA metabarcoding reservoir PLOS ONE | https://doi.org/10.1371/journal.pone.0314210 January 24, 2025 10 / 22