LIFEPLAN: A worldwide biodiversity sampling design

Sampling methods Cameras and audio recorders collected data continuously year-round with weekly visits needed to change batteries and collect the data manually. Camera. The protocol described in this peer-reviewed article is published on protocols.io, dx.doi.org/10.17504/protocols.io.q26g7pxp1gwz/v3 and is included for printing as S1 File with this article. Audio. The protocol described in this peer-reviewed article is published on protocols.io, dx.doi.org/10.17504/protocols.io.kqdg3xbp1g25/v2 and is included for printing as S2 File with this article. Two 24-hour cyclone samples are collected while weather conditions allow the sampler to work, by visiting the site on three consecutive days each week or by using a timer for one of the samples and visiting the site two consecutive days per week. In a few cases, only one sample is collected using a timer due to the difficulty of reaching the site multiple days per week. The protocol described in this peer-reviewed article is published on protocols.io, dx.doi.org/10. 17504/protocols.io.6qpvr3zn2vmk/v1 and is included for printing as S3 File with this article. Flying arthropod samples are collected from Malaise traps once per week during the insect flying season producing a seven-day sample. The protocol described in this peer-reviewed arti- cle is published on protocols.io, dx.doi.org/10.17504/protocols.io.kqdg3xkdqg25/v2 and is included for printing as S4 File with this article. One soil sample from each of three or five points defined by the camera and audio recorder

locations is taken eight or four times per year when the temperatures are above freezing. The original frequency of eight times a year was reduced to four times a year in January 2023. The protocol described in this peer-reviewed article is published on protocols.io, dx.doi.org/10. 17504/protocols.io.5jyl8pw9rg2w/v2 and is included for printing as S5 File with this article. Fig 6. Two alternate LIFEPLAN site designs. (A) Design of LIFEPLAN one-hectare site with five sampling points. (B) Design of LIFEPLAN transect with three sampling points. Cameras and AudioMoths are placed within 10 m of each sampling point. Soil samples are collected in an area within approximately 5 m of the camera and audio recorder. The Malaise trap and cyclone sampler can be placed anywhere within the site, preferably close to the centre or middle but not in the camera’s field of view. https://doi.org/10.1371/journal.pone.0313353.g006 PLOS ONE LIFEPLAN: A worldwide biodiversity sampling design PLOS ONE | https://doi.org/10.1371/journal.pone.0313353 December 31, 2024 8 / 15