Enhanced biodiversity of gut flora and feed efficiency in pond cultured tilapia under reduced frequency feeding strategies

S1 Table. Primers used in studies for Nile tilapia (O. niloticus) subjected to reduced-feed- ing regimens. (XLSX) S2 Table. Water quality parameters of ponds used in studies for Nile tilapia (O. niloticus) subjected to reduced-feeding regimens. Values are mean ± SD. Values with different letters are significantly different (P < 0.05). (XLSX) S3 Table. Plankton abundance in ponds used in studies for Nile tilapia (O. niloticus) sub- jected to reduced-feeding regimens. Values are mean ± SEM. Values with different letters are significantly different (P < 0.05). (XLSX) S4 Table. Proportions by treatment of prokaryotes and eukaryotes identified to the family level in fecal material from Nile tilapia subjected to reduced-feeding regimens. (XLSX) S5 Table. Unique prokaryotic Operational Taxonomic Units (OTUs) identified in the fecal material from Nile tilapia subjected to reduced-feeding regimens. (XLSX) Acknowledgments The authors would like to thank Dr. Hillary Egna of Oregon State University for her leadership of the Aquafish Innovation Lab and her intellectual contributions to the work. The opinions expressed herein are those of the authors and do not necessarily reflect those of the AquaFish Innovation Lab-USAID or the NSF. Author Contributions Conceptualization: Md. Abdul Wahab, David A. Baltzegar, Russell J. Borski. Data curation: Scott A. Salger. Formal analysis: Scott A. Salger. Funding acquisition: Md. Abdul Wahab, Russell J. Borski. Investigation: Jimi Reza, Md. Abdul Wahab, Alexander T. Murr, Russell J. Borski.

Methodology: Scott A. Salger, David A. Baltzegar, Alexander T. Murr. Project administration: Md. Abdul Wahab, Russell J. Borski. Resources: Md. Abdul Wahab, Russell J. Borski. Supervision: Md. Abdul Wahab, Russell J. Borski. Validation: Scott A. Salger. Visualization: Scott A. Salger. Writing – original draft: Scott A. Salger. Writing – review & editing: Scott A. Salger, Courtney A. Deck, Russell J. Borski. PLOS ONE Enhanced biodiversity of gut flora and feed efficiency in pond cultured tilapia PLOS ONE | https://doi.org/10.1371/journal.pone.0236100 July 23, 2020 19 / 23