Hidden biodiversity in Neotropical streams: DNA barcoding uncovers high endemicity of freshwater macroinvertebrates at small spatial scales

S3 Fig. Phylogenetic tree determined by the Maximum Likelihood (ML). Data represent cox1 sequences obtained from 224 freshwater macroinvertebrates collected within the Panama Canal Watershed. The numbers on the branches show nodal support. (PNG) S1 Table. Molecular and taxonomic diversity of freshwater macroinvertebrates within the Panama Canal Watershed. For each specimen, we show a taxonomic group (i.e., order, fam- ily, and genus), molecular species identity (MOTUs: based on ABGD and GMYC), Haplotype identity, Genbank accession number, and sampling site. Sites are Trinidad (TRI), Frijolito (FTO), Frijoles (FES) and Indio (IND). (XLSX) Acknowledgments We dedicate this study to our friend Ruth G. Reina. Her passion for tropical freshwater biology served as an inspiration to this work. Logistical support was provided by the Smithsonian Tropical Research Institute. Field assistance was provided by Celestino Martı´nez, Nohelys Alvarado, Carlos Nieto, and De´bora Delgado. Diana Sharpe provided valuable comments on an earlier version of the manuscript. We thank editor Dr. Michael A. Chadwick and four anonymous reviewers for providing feedback on our manuscript. Author Contributions Conceptualization: Luis F. De Leo´n. Data curation: Luis F. De Leo´n, Aydee´ Cornejo, Ronnie G. Gavila´n, Celestino Aguilar. Formal analysis: Luis F. De Leo´n, Ronnie G. Gavila´n, Celestino Aguilar. Funding acquisition: Luis F. De Leo´n. Investigation: Luis F. De Leo´n, Aydee´ Cornejo, Ronnie G. Gavila´n, Celestino Aguilar. Methodology: Luis F. De Leo´n, Aydee´ Cornejo, Ronnie G. Gavila´n.

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