Molecular diet studies of water mites reveal prey biodiversity

Fig 4. Neighbor-joining tree of non-mite DNA OTUs in representative Lebertia quinquemaculosa mite of PCR products amplified with mLep/LCOI primers-Illumina adapter primers. Each branch is labeled with the “best hit” accession ID in GenBank, taxon name, and the percent identity of the OTU to its best hit. Mostly chironomid and mosquito DNA in a specimen of L. quinquemaculosa. Color code: red, identities >96.5%; blue, identities 90% - 96.5%; black, identities 80% - 90%. All OTUs represent >10 sequences. https://doi.org/10.1371/journal.pone.0254598.g004 Fig 5. Neighbor-joining tree of non-mite DNA OTUs in representative Lebertia quinquemaculosa mites of PCR products amplified with mLep/LCOI primers-Illumina adapter primers. Each branch is labeled with the “best hit” accession ID in GenBank, taxon name, and the percent identity of the OTU to its best hit. A & B, A mix of oligochaetes and chironomids in specimens of L. quinquemaculosa. Color code: red, identities >96.5%; blue, identities 90% - 96.5%; black, identities 80% - 90%. All OTUs represent >4 sequences in A and >10 sequences in B. https://doi.org/10.1371/journal.pone.0254598.g005 PLOS ONE Prey DNA diversity in water mites PLOS ONE | https://doi.org/10.1371/journal.pone.0254598 July 29, 2021 9 / 18