Molecular diet studies of water mites reveal prey biodiversity

Acknowledgments We thank the Sharon L. Ram Aquatic Sciences Foundation, Dr. David Cook, Fred A. and Bar- bara M. Erb Family Foundation, the Belle Isle Conservancy for use of the Belle Isle Aquarium Field Research Laboratory; Shlomo Sawilowsky of the College of Education, Wayne State Uni- versity for assistance with statistics; and Xavier Walker, Milad Qazazi, Janiel Cruz and Tiffany Burris for assistance in animal collections and PCR. We give thanks to two reviewers who have helped to greatly improve this manuscript. Author Contributions Conceptualization: Adrian A. Vasquez, Jeffrey L. Ram. Data curation: Adrian A. Vasquez, Obadeh Mohiddin, Zeyu Li, Brittany L. Bonnici, Katherine Gurdziel, Jeffrey L. Ram. Formal analysis: Adrian A. Vasquez, Obadeh Mohiddin, Zeyu Li, Brittany L. Bonnici, Kather- ine Gurdziel, Jeffrey L. Ram. Funding acquisition: Adrian A. Vasquez, Jeffrey L. Ram. Investigation: Adrian A. Vasquez, Jeffrey L. Ram. Methodology: Adrian A. Vasquez, Obadeh Mohiddin, Zeyu Li, Brittany L. Bonnici, Katherine Gurdziel, Jeffrey L. Ram. Writing – original draft: Adrian A. Vasquez, Jeffrey L. Ram. Writing – review & editing: Adrian A. Vasquez, Katherine Gurdziel, Jeffrey L. Ram. References 1. Di Sabatino A, Smit H, Gerecke R, Goldschmidt T, Matsumoto N, Cicolani B. Global diversity of water mites (Acari, Hydrachnidia; Arachnida) in freshwater. Hydrobiologia. 2008; 595:303–15. https://doi.org/ 10.11646/zootaxa.3720.1.1 PMID: 26079004 2. Vasquez AA, Qazazi MS, Fisher JR, Failla AJ, Rama S, Ram JL. New molecular barcodes of water

mites (Trombidiformes: Hydrachnidiae) from the Toledo Harbor region of Western Lake Erie, USA, with first barcodes for Krendowskia (Krendowskiidae) and Koenikea (Unionicolidae). International Journal of Acarology. 2017; 43(6):494–8. https://doi.org/10.1080/01647954.2017.1349178 WOS:000407480100012. 3. Christoffersen ML. Continental biodiversity of South American oligochaetes: The importance of invento- ries. Acta Zoologica Mexicana Nueva Serie. 2010; 26(Sp. Iss. 2):35–46. BCI:BCI201100225979. 4. Strayer DL. Challenges for freshwater invertebrate conservation. Journal of the North American Bentho- logical Society. 2006; 25(2):271–87. https://doi.org/10.1899/0887-3593(2006)25[271:Cffic]2.0.Co;2 WOS:000237891600002. 5. Proctor H, Pritchard G. Neglected predators—water mites (acari, parasitengona, hydrachnellae) in fresh-water communities. Journal of the North American Benthological Society. 1989; 8(1):100–11. https://doi.org/10.2307/1467406 WOS:A1989T750400010. 6. Ten Winkel EH, Davids C, De Nobel JG. Food and feeding strategies of water mites of the genus Hygro- bates and the impact of their predation on the larval population of the chironomid Cladotanytarsus man- cus (Walker) in Lake Maarsseveen. Netherlands Journal of Zoology. 1989; 39(3–4):246–63. 7. Mwango J, Williams T, Wiles R. A preliminary study of the predator-prey relationships of watermites (Acari: Hydrachnidia) and blackfly larvae (Diptera: Simuliidae). Entomologist. 1995; 114(2):107–17. BCI:BCI199598373281.

8. Smith IM, Cook DR, Smith BP. Ch. 15, Water mites and other arachnids. In: Thorpe J, Covich A, editors. Ecology and classification of North American freshwater invertebrates 3rd Edition Waltham, Massa- chusetts: Academic Press; 2010. p. pp. 485–586. 9. Matveev VF, Martinez CC. Can water mites control populations of planktonic cladocera? Hydrobiologia. 1990; 198:227–31. https://doi.org/10.1007/bf00048637 WOS:A1990DR42200020. PLOS ONE Prey DNA diversity in water mites PLOS ONE | https://doi.org/10.1371/journal.pone.0254598 July 29, 2021 16 / 18