The Himalayan uplift and evolution of aquatic biodiversity across Asia: Snowtrout (Cyprininae: Schizothorax) as a test case

Fig 4. Maximum likelihood (ML) tree reflecting phylogenetic relationships among biogeographic samples. The constituent samples representing each biogeographic clade are provided in S3 Table under ’Species Name,’ ’Haplotype,’ ’Accession #,’ ’Location,’ and ’Group’ headings.’ Data were derived from sequence analysis of the cytochrome-b mitochondrial gene (1,140 bp, 140 distinct haplotypes). Red asterisk = SH-ALRT (Shimodaira-Hasegawa approximate likelihood ratio test) � 80% and UFBOOT (ultrafast bootstrap approximations) � 95% (IQ-TREE v.2 Manual: http://www. PLOS ONE Himalayan uplift and aquatic evolution PLOS ONE | https://doi.org/10.1371/journal.pone.0289736 October 24, 2023 13 / 27