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Effects of woody forages on biodiversity and bioactivity of aerobic culturable gut bacteria of tilapia (Oreochromis niloticus)

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results contribute to our knowledge and understanding of the intestinal bacterial diversity and bioactivity of tilapia and can aid in the further exploitation of new diets and probiotic source in aquaculture. Author Contributions Conceptualization: Xiaoyong Zhang, Qing Pan. Data curation: Biao Chen. Methodology: Feng Wu. Writing – review & editing: Sha Liu, Xiongjian Xia, Liuling Gao. References 1. He S, Zhou Z, Liu Y, et al. Effects of the antibiotic growth promoters flavomycin and florfenicol on the autochthonous intestinal microbiota of hybrid tilapia (Oreochromis niloticus♀× O. aureus♂). Archives of microbiology. 2010; 192(12): 985–994. https://doi.org/10.1007/s00203-010-0627-z PMID: 20844867 2. Kaktcham PM, Piame LT, Sileu GMS, et al. Bacteriocinogenic Lactococcus lactis subsp. lactis 3MT iso- lated from freshwater Nile Tilapia: isolation, safety traits, bacteriocin characterisation, and application for biopreservation in fish paˆ te´ . Archives of microbiology. 2019: 1–10. 3. Chiu A, Li L, Guo S, et al. Feed and fishmeal use in the production of carp and tilapia in China. Aquacul- ture. 2013; 414: 127–134. 4. Moutinho S, Martı´nez-Llorens S, Toma´s-Vidal A, et al. Meat and bone meal as partial replacement for fish meal in diets for gilthead seabream (Sparus aurata) juveniles: Growth, feed efficiency, amino acid utilization, and economic efficiency. Aquaculture. 2017; 468: 271–277. 5. Anderson A, Alam M, Watanabe W, et al. Full replacement of menhaden fish meal protein by low-gossy-

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pol cottonseed flour protein in the diet of juvenile black sea bass Centropristis striata. Aquaculture. 2016; 464: 618–628. 6. Bian W, Wang LG, Zhang HZ, et al, editors. Study on phosphorus pollution load of aquaculture in Baiyangdian Lake. Advanced Materials Research; 2012: Trans Tech Publ. 7. Torrecillas S, Robaina L, Caballero M, et al. Combined replacement of fishmeal and fish oil in European sea bass (Dicentrarchus labrax): production performance, tissue composition and liver morphology. Aquaculture. 2017; 474: 101–112. 8. Priyadarshini M, Manissery JK, Gangadhara B, et al. Growth response of Catla catla (Actinopterygii: Cypriniformes: Cyprinidae) to soya and maize supplemented traditional feed mixture. Acta Ichthyolo- gica Et Piscatoria. 2011; 41(3): 159–164. 9. Hu Y, Huang Y, Feng F, et al. Effect of soybean meal replacement by cottonseed meal on growth, feed utilization and some blood physiological/biochemical indices of juvenile black carp, Mylopharyngodon piceus. Aquaculture research. 2015; 46(10): 2490–2500. 10. Silva RLd, Damasceno FM, Rocha MKHR, et al. Replacement of soybean meal by peanut meal in diets for juvenile Nile tilapia, Oreochromis niloticus. Latin american journal of aquatic research. 2017; 45(5): 1044–1053. 11. Solomon S, Okomoda V, Onah R. Nutritional profile of soaked Cajanus cajan (L.) Millsp. and its utiliza- tion as partial replacement for soybean meal in the diet of Clarias gariepinus (Burchell, 1822) finger- lings. Journal of Applied Ichthyology. 2017; 33(3): 450–457.

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12. Yuangsoi B, Masumoto T. Replacing moringa leaf (Moringa oleifera) partially by protein replacement in soybean meal of fancy carp (Cyprinus carpio). Songklanakarin Journal of Science & Technology. 2012; 34(5). 13. Moyo B, Masika PJ, Hugo A, et al. Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. African Journal of Biotechnology. 2011; 10(60): 12925–12933. 14. Katsube T, Imawaka N, Kawano Y, et al. Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food chemistry. 2006; 97(1): 25–31. 15. Sun J, Liu S-f, Zhang C-s, et al. Chemical composition and antioxidant activities of Broussonetia papyri- fera fruits. PloS one. 2012; 7(2): e32021. https://doi.org/10.1371/journal.pone.0032021 PMID: 22389678 16. Ru-gang H. Application Effects of Moringa Leaf Powder in Fish Feed. Journal of Anhui Agricultural Sci- ences. 2013; 4. PLOS ONE Effects of wood forages on gut bacterial biodiversity and bioactivity in tilapia PLOS ONE | https://doi.org/10.1371/journal.pone.0235560 July 2, 2020 14 / 17