Shifts in biodiversity and physical structure of seagrass beds across 5 decades at Carriacou, Grenadines

14. Unsworth RK, Collier CJ, Henderson GM, McKenzie LJ. Tropical seagrass meadows modify seawater carbon chemistry: implications for coral reefs impacted by ocean acidification. Environ. Res. Lett. 2012; 7: 024026. https://doi.org/10.1088/1748-9326/7/2/024026 15. Bergstrom E, Silva J, Martins C, Horta P. Seagrass can mitigate negative ocean acidification effects on calcifying algae. Sci Rep. 2019; 9: 1–11. https://doi.org/10.1038/s41598-018-35670-3 16. Lovelock CE, Duarte CM. Dimensions of blue carbon and emerging perspectives. Biol Lett. 2019; 15: 20180781. https://doi.org/10.1098/rsbl.2018.0781 PMID: 30836882 17. Mcleod E, Chmura GL, Bouillon S, Salm R, Bjo¨rk M, Duarte CM, et al. A blueprint for blue carbon: toward an improved understanding of the role of vegetated coastal habitats in sequestering CO2. Front Ecol Environ. 2011; 9: 552–560. https://doi.org/10.1890/110004 18. Gallagher AJ, Brownscombe JW, Alsudairy NA, Casagrande AB, Fu C, Harding L, et al. Tiger sharks support the characterization of the world’s largest seagrass ecosystem. Nat Commun. 2022; 13: 6328. https://doi.org/10.1038/s41467-022-33926-1 PMID: 36319621 19. Johannessen SC. How can blue carbon burial in seagrass meadows increase long-term, net sequestra- tion of carbon? A critical review. Environ Res Lett. 2022; 17: 093004. https://doi.org/10.1088/1748- 9326/ac8ab4 20. Dunic JC, Brown CJ, Connolly RM, Turschwell MP, Coˆ te´ IM. Long-term declines and recovery of meadow area across the world’s seagrass bioregions. Global Change Biol. 2021; 17: 4096–4109.

https://doi.org/10.1111/gcb.15684 PMID: 33993580 21. Waycott M, Duarte CM, Carruthers TJ, Orth RJ, Dennison WC, Olyarnik S, et al. Accelerating loss of seagrasses across the globe threatens coastal ecosystems. Proc Natl Acad Sci USA. 2009; 106: 12377–81. https://doi.org/10.1073/pnas.0905620106 PMID: 19587236 22. van Tussenbroek BI, Corte´s J, Collin R, Fonseca AC, Gayle PM, Guzma´n HM, et al. Caribbean-wide, long-term study of seagrass beds reveals local variations, shifts in community structure and occasional collapse. PloS ONE. 2014; 9:e90600. https://doi.org/10.1371/journal.pone.0090600 PMID: 24594732 23. Leo´ n-Pe´ rez MC, Armstrong RA, Herna´ ndez WJ, Aguilar-Perera A, Thompson-Grim J. Seagrass cover expansion off Caja de Muertos Island, Puerto Rico, as determined by long-term analysis of historical aerial and satellite images (1950–2014). Ecol Indic. 2020; 117: 106561. https://doi.org/10.1016/j. ecolind.2020.106561 24. Tomasko D, Alderson M, Burnes R, Hecker J, Leverone J, Raulerson G, et al. Widespread recovery of seagrass coverage in Southwest Florida (USA): temporal and spatial trends and management actions responsible for success. Mar Pollut Bull. 2018; 135: 1128–1137. https://doi.org/10.1016/j.marpolbul. 2018.08.049 PMID: 30301011 25. Herna´ndez-Delgado EA, Toledo-Herna´ ndez C, Ruı´z-Dı´az CP, Go´ mez-Andu´jar N, Medina-Muñiz JL, Canals-Silander MF, et al. Hurricane impacts and the resilience of the invasive sea vine, Halophila sti- pulacea: A case study from Puerto Rico. Estuaries Coasts. 2020; 43: 1263–1283. https://doi.org/10. 1007/s12237-019-00673-4

26. Ruiz H, Ballantine DL. Occurrence of the seagrass Halophila stipulacea in the tropical west Atlantic. Bull. Mar. Sci. 2004; 75: 131–135. 27. James RK, Keyzer LM, van de Velde SJ, Herman PM, van Katwijk MM, Bouma TJ. Climate change miti- gation by coral reefs and seagrass beds at risk: How global change compromises coastal ecosystem services. Sci Total Environ. 2023; 857: 159576. https://doi.org/10.1016/j.scitotenv.2022.159576 PMID: 36273559 28. Schuerch M, Spencer T, Temmerman S, Kirwan ML, Wolff C, Lincke D, et al. Future response of global coastal wetlands to sea-level rise. Nature. 2018; 56: 231–234. https://doi.org/10.1038/s41586-018- 0476-5 PMID: 30209368 29. de Paula Costa MD, Lovelock CE, Waltham NJ, Young M, Adame MF, Bryant CV, et al. Current and future carbon stocks in coastal wetlands within the Great Barrier Reef catchments. Glob Chang Biol. 2021; 14:3257–3271. https://doi.org/10.1111/gcb.15642 30. Jevrejeva S, Bricheno L, Brown J, Byrne D, De Dominicis M, Matthews A, et al. Quantifying processes contributing to marine hazards to inform coastal climate resilience assessments, demonstrated for the Caribbean Sea. Nat Hazards Earth Syst Sci. 202; 20: 2609–2626. https://doi.org/10.5194/nhess-20- 2609-2020 31. Saunders MI, Leon JX, Callaghan DP, Roelfsema CM, Hamylton S, Brown CJ, et al. Interdependency of tropical marine ecosystems in response to climate change. Nature Clim Change. 2014; 4: 724–729. https://doi.org/10.1038/nclimate2274 32. Keyzer LM, Herman PM, Smits BP, Pietrzak JD, James RK, Candy AS, et al. The potential of coastal

ecosystems to mitigate the impact of sea-level rise in shallow tropical bays. Estuar Coast Shelf Sci. 2020; 246: 107050. https://doi.org/10.1016/j.ecss.2020.10705033 PLOS ONE Seagrass bed biodiversity and structure and across 5 decades PLOS ONE | https://doi.org/10.1371/journal.pone.0306897 August 1, 2024 22 / 25