Unveiling biogeographical patterns of the ichthyofauna in the Tuichi basin, a biodiversity hotspot in the Bolivian Amazon, using environmental DNA

Fig 1. Map of the sampling locations. Map A shows the location of the 34 sites sampled and Map B focuses on sites south of the 13th parallel. The lake sites are represented by yellow disks (labelled with their full name), river sites are represented according to their hydrographic rank 1, 2 and 3 by red diamonds (samples 1–2), red squares (samples 3–17) and red triangles (samples a-g), respectively. Samples 6 and 7, as well as 12 and 13, were carried out at two sites on the Tuichi river in 2018 and 2019, respectively. Maps were created with QGIS v3.4 using the base map Stamen™ Terrain Background. The points were shifted for better viewing, but the actual positions are reported in the S1 Table. The hydro-ecoregions (reprinted from Wasson et al. 2002 under a CC BY license, with permission from International Association of Hydrological Sciences, original copyright 2002) were also indicated (see legend for details). https://doi.org/10.1371/journal.pone.0262357.g001 PLOS ONE Fish diversity and distribution in the Tuichi basin PLOS ONE | https://doi.org/10.1371/journal.pone.0262357 January 4, 2022 4 / 25