Unveiling biogeographical patterns of the ichthyofauna in the Tuichi basin, a biodiversity hotspot in the Bolivian Amazon, using environmental DNA

S4 Fig. NMDS ordination (site names plotted). (PDF) S5 Fig. NMDS ordination including all sites. Orders are split into different facets. (PDF) S6 Fig. Species dendrogram (binary Jaccard distance and Ward2 aggregation). (PDF) S1 Text. Scripts and command lines used for data analysis and plotting figures. (PDF) S2 Text. Assessment of the taxonomic resolution of markers used on Amazonian fish. (PDF) S1 Table. Informations on sites and points of collect (date, collected volume, depth, coor- dinates). (XLSX) S2 Table. COI fish database used (fasta file). (XLSX) S3 Table. Environmental variables (soils, altitude, coordinate, geology, richness, Jack2. . .). (XLSX) S4 Table. Sequencing results per library. (XLSX) S5 Table. Taxa abundance per library. (XLSX) S6 Table. Quality of assignation (%ID) and list of species assigned to affinis status. (XLSX) S7 Table. List of taxa present in each area of the Venn diagram constructed at the cluster level. (XLSX) Acknowledgments The authors acknowledge the IRD itrop HPC (South Green Platform) at IRD Montpellier for providing HPC resources that contributed to the research results reported within this paper (URL: https://bioinfo.ird.fr/- http://www.southgreen.fr). Author Contributions Conceptualization: Ce´dric Mariac, Fabrice Duponchelle, Jean-Franc¸ois Renno. Formal analysis: Ce´dric Mariac, Fabrice Duponchelle, Jean-Franc¸ois Renno. Methodology: Ce´dric Mariac, Guido Miranda, Camila Ramallo, Gabriel Tarifa, Jean-Franc¸ois Renno. Validation: Ce´dric Mariac. Writing – original draft: Ce´dric Mariac, Fabrice Duponchelle, Jean-Franc¸ois Renno.

PLOS ONE Fish diversity and distribution in the Tuichi basin PLOS ONE | https://doi.org/10.1371/journal.pone.0262357 January 4, 2022 20 / 25