Unveiling biogeographical patterns of the ichthyofauna in the Tuichi basin, a biodiversity hotspot in the Bolivian Amazon, using environmental DNA

Estimation of eDNA detection distances in streams and lakes. We assessed the minimum dis- tance from which detectability is no longer possible in the environmental context (dry season) of our sampling. For this, we used the situation where taxa were present at a site, but not Fig 3. Maps showing taxon richness in the study area. For lakes (blue discs) the intensity of the colour is proportional to the observed richness (Mentiroso and Tumichucua lakes in the smaller box A). River sites are represented according to their hydrographic rank 1, 2 and 3 by diamonds, squares and triangles, respectively and a colour code associated with the biogeographic entities (BE) defined by the hierarchical analysis. The observed richness in rivers was projected and extrapolated on the hydrological network and represented by the colour code provided in the legend (network in red is not extrapolated). https://doi.org/10.1371/journal.pone.0262357.g003 PLOS ONE Fish diversity and distribution in the Tuichi basin PLOS ONE | https://doi.org/10.1371/journal.pone.0262357 January 4, 2022 11 / 25