Genetic variability in microbial eukaryotes reshapes marine biodiversity assessment in the age of amplicon sequencing

PLOS One | https://doi.org/10.1371/journal.pone.0326053 June 20, 2025 4 / 29 Fig 1. Number of ASVs per specimen among three orders of polycystine Radiolaria. Each dot represents one specimen; panels show the distribu- tion of ASVs per specimen in Collodaria (pink), Nassellaria (brown), Spumellaria (green), and all taxa combined (gray). These include: the total number of ASVs that passed all vetting procedures described in “Materials and methods” (a); the subset of ASVs which constituted at least 0.1% of the number of reads per specimen (b); ASVs that made up at least 1% of reads per specimen (c); and ASVs comprising at least 10% read abundance per specimen (d). Panels (e–h) display corresponding intragenomic richness information for only the extracted V4 regions of the full-length ASVs, respectively. Box- plots denote the median, interquartile range, maximum, and minimum values (outliers excluded). All data underlying this figure is provided in S1 Data. https://doi.org/10.1371/journal.pone.0326053.g001