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Cumulative pre-cooling methods do not enhance cycling performance in tropical climate

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Data curation: Aure´lie Collado, Ke´vin Rinaldi. Formal analysis: Aure´lie Collado, Ke´vin Rinaldi. Funding acquisition: Olivier Hue. Investigation: Aure´lie Collado, Ke´vin Rinaldi, Eric Hermand. Methodology: Aure´lie Collado, Ke´vin Rinaldi, Eric Hermand. Project administration: Ke´vin Rinaldi, Olivier Hue. Resources: Ke´vin Rinaldi. Software: Aure´lie Collado. Supervision: Olivier Hue. Validation: Olivier Hue. Writing – original draft: Aure´lie Collado, Ke´vin Rinaldi. Writing – review & editing: Eric Hermand. References 1. Voltaire B, Berthouze-Aranda S, Hue O. Influence of a hot/wet environment on exercise performance in natives to tropical climate. J Sports Med Phys Fitness. 2003; 43: 306–311. PMID: 14625511 2. Hue O. The challenge of performing aerobic exercise in tropical environments: Applied knowledge and perspectives. Int J Sports Physiol Perform. 2011; 6: 443–454. https://doi.org/10.1123/ijspp.6.4.443 PMID: 22248546 3. Maughan RJ, Otani H, Watson P. Influence of relative humidity on prolonged exercise capacity in a warm environment. Eur J Appl Physiol. 2012; 112: 2313–2321. https://doi.org/10.1007/s00421-011- 2206-7 PMID: 22012542 4. Che Muhamed AM, Atkins K, Stannard SR, Mu¨ ndel T, Thompson MW. The effects of a systematic increase in relative humidity on thermoregulatory and circulatory responses during prolonged running exercise in the heat. Temperature. 2016; 3: 455–464. https://doi.org/10.1080/23328940.2016.1182669 PMID: 28349085 5. Jones PR, Barton C, Morrissey D, Maffulli N, Hemmings S. Pre-cooling for endurance exercise perfor-

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mance in the heat: a systematic review. BMC Med. 2012; 10: 166. https://doi.org/10.1186/1741-7015- 10-166 PMID: 23249542 6. Tyler CJ, Sunderland C, Cheung SS. The effect of cooling prior to and during exercise on exercise per- formance and capacity in the heat: a meta-analysis. Br J Sports Med. 2015; 49: 7–13. https://doi.org/10. 1136/bjsports-2012-091739 PMID: 23945034 7. Bongers CCWG, Thijssen DHJ, Veltmeijer MTW, Hopman MTE, Eijsvogels TMH. Precooling and per- cooling (cooling during exercise) both improve performance in the heat: A meta-analytical review. Br J Sports Med. 2015; 49: 377–384. https://doi.org/10.1136/bjsports-2013-092928 PMID: 24747298 8. Bongers CCWG, Hopman MTE, Eijsvogels TMH. Cooling interventions for athletes: An overview of effectiveness, physiological mechanisms, and practical considerations. Temperature. 2017; 4: 60–78. https://doi.org/10.1080/23328940.2016.1277003 PMID: 28349095 9. Lee JKW, Shirreffs SM, Maughan RJ. Cold drink ingestion improves exercise endurance capacity in the heat. Med Sci Sports Exerc. 2008; 40: 1637–1644. https://doi.org/10.1249/MSS.0b013e318178465d PMID: 18685527 10. Ross MLR, Garvican LA, Jeacocke NA, Laursen PB, Abbiss CR, Martin DT, et al. Novel precooling strategy enhances time trial cycling in the heat. Med Sci Sports Exerc. 2011; 43: 123–133. https://doi. org/10.1249/MSS.0b013e3181e93210 PMID: 20508537 11. Osakabe J, Matsumoto T, Umemura Y. Ice slurry ingestion as a cooling strategy in the heat. JPFSM. 2019; 8: 73–78. https://doi.org/10.7600/jpfsm.8.73

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12. U¨ ckert S, Joch W. Effects of warm-up and precooling on endurance performance in the heat. Br J Sports Med. 2007; 41: 380–384. https://doi.org/10.1136/bjsm.2006.032292 PMID: 17224434 PLOS ONE Tropical cycling performance and cumulative pre-cooling PLOS ONE | https://doi.org/10.1371/journal.pone.0291951 October 12, 2023 13 / 15