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Biodiversity and disease risk in an algal biofuel system: An experimental test in outdoor ponds using a before-after-control-impact (BACI) design

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Supporting information S1 Data. Co-optima data. (XLSX) S1 Appendix. (DOCX) Acknowledgments We would like to acknowledge the valuable feedback provided by the anonymous reviewers, for which the manuscript is greatly improved. Author Contributions Conceptualization: Bradley J. Cardinale. Data curation: Spenser L. Widin. Formal analysis: Spenser L. Widin. Funding acquisition: Bradley J. Cardinale. Investigation: Spenser L. Widin, Kia M. Billings. Methodology: Bradley J. Cardinale. Project administration: Bradley J. Cardinale. Resources: John McGowen. Supervision: Bradley J. Cardinale. Writing – original draft: Spenser L. Widin. Writing – review & editing: Spenser L. Widin, Kia M. Billings, John McGowen, Bradley J. Cardinale. References 1. Borowitzka MA, Moheimani NR. Sustainable biofuels from algae. Mitigation and Adaptation Strategies for Global Change. 2013; 18(1):13–25. https://doi.org/10.1007/s11027-010-9271-9 2. Chisti Y. Biodiesel from microalgae. Biotechnology Advances. 2007; 25(3):294–306. https://doi.org/10. 1016/j.biotechadv.2007.02.001 PMID: 17350212 3. Chisti Y. Biodiesel from microalgae beats bioethanol. Trends in Biotechnology. 2008; 26(3):126–31. https://doi.org/10.1016/j.tibtech.2007.12.002 PMID: 18221809 4. Raheem A, Prinsen P, Vuppaladadiyam AK, Zhao M, Luque R. A review on sustainable microalgae based biofuel and bioenergy production: Recent developments. Journal of Cleaner Production. 2018; 181:42–59. https://doi.org/10.1016/j.jclepro.2018.01.125.

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5. Georgianna DR, Mayfield SP. Exploiting diversity and synthetic biology for the production of algal biofu- els. NATURE. 2012; 488(7411):329–35. https://doi.org/10.1038/nature11479 PMID: 22895338 6. Carney LT, Lane TW. Parasites in algae mass culture. FRONT MICROBIOL. 2014; 5:278–. https://doi. org/10.3389/fmicb.2014.00278 PMID: 24936200 7. Schenk PM, Thomas-Hall SR, Stephens E, Marx UC, Mussgnug JH, Posten C, et al. Second Genera- tion Biofuels: High-Efficiency Microalgae for Biodiesel Production. BioEnergy Research. 2008; 1(1):20– 43. https://doi.org/10.1007/s12155-008-9008-8 8. Sheehan J, Dunahay T, Benemann J, Roessler P. Look Back at the U.S. Department of Energy’s Aquatic Species Program: Biodiesel from Algae; Close-Out Report. United States: 1998 1998-07-01. Report No. PLOS ONE Biodiversity, biofuel and disease: Ecological engineering in algal feedstocks PLOS ONE | https://doi.org/10.1371/journal.pone.0267674 April 28, 2022 13 / 15