Screening and biodiversity analysis of cultivable inorganic phosphate–solubilizing bacteria in the rhizosphere of Hydrilla verticillata

According to the method described in a previous research [23], among 28 IPB strains screened, strains with greater than 98.65% 16S rRNA gene sequence similarity are classified as belonging to the same species. Therefore, strains SWIH-7, SWIP-6, SWIP-7, SWIP-13, SWIP- 15 and SWIP-16 are tentatively identified as potential new species. All 16S rRNA sequences of representative strains are selected from Table 1 for BLAST anal- ysis on the NCBI website to search for sequences with high homology and construct phyloge- netic tree. According to Fig 1, the evolutionary tree is divided into two large branches, and Table 1. (Continued) Strain Number Similar Strain a Gene Identity (%) b Identification Results Registry Number c SWIH-9 Priestia aryabhattai B8W22 (MTCC:7755;JCM:13839) 99.72 Priestia aryabhattai ON479649 Note: a. Sequence with highest percentage of identity observed in EzBioCloud. b. Percentage of identity with EzBioCloud analysis. c. The accession number of IPB in NCBI. https://doi.org/10.1371/journal.pone.0297047.t001 Fig 1. Phylogenetic evolutionary tree constructed based on 16S rRNA gene sequences. The numbers at the nodes indicate the bootstrap values based on the neighbor-joining analyses of 1000 resampled data sets. The scale bar indicates evolutionary distance. https://doi.org/10.1371/journal.pone.0297047.g001 PLOS ONE Screening and biodiversity analysis of cultivable inorganic phosphate–solubilizing bacteria PLOS ONE | https://doi.org/10.1371/journal.pone.0297047 January 19, 2024 6 / 11