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Screening and biodiversity analysis of cultivable inorganic phosphate–solubilizing bacteria in the rhizosphere of Hydrilla verticillata

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Author Contributions Conceptualization: Yong Li. Data curation: Xintao Yu, Sidan Gong, Zhilian Gong. Formal analysis: Sidan Gong, Zhilian Gong. Funding acquisition: Yong Li. Investigation: Huan Liu, Xintao Yu. Methodology: Huan Liu, Xintao Yu. Project administration: Yong Li. Supervision: Yong Li, Zhilian Gong. Visualization: Sidan Gong, Zhilian Gong. Writing – original draft: Yong Li, Huan Liu. Writing – review & editing: Yong Li, Huan Liu. References 1. Zamparas M, Zacharias I. Restoration of eutrophic freshwater by managing internal nutrient loads: a review. Sci Total Environ. 2014; 496: 551–562. https://doi.org/10.1016/j.scitotenv.2014.07.076 PMID: 25108796 2. Guo Y, Qin YY, Ju TC, Leng S, Wu D, Li MT. Isolation and characterization of a strain endophytic bacte- rium in Myriophyllum spicatum and its solubilization of phosphate. Acta Scientiae Circumstantiae. 2016; 36: 4352–4360. https://doi.org/10.13671/j.hjkxxb.2016.0174 3. Wei WW, Li CH, Ye C, Dai WQ, Zheng PR, Huang XY, et al. Effect of Decomposition on Nitrogen Migra- tion and Transformation in Water-Sediment-Hydrilla verticillata Royle Macrophytes System. Environ Sci Technol. 2019; 42: 1–9. https://doi.org/10.19672/j.cnki.1003-6504.2019.07.001 4. Coetzee JA, Hill MP, Schlange D. Potential spread of the invasive plant Hydrilla verticillata in South Africa based on anthropogenic spread and climate suitability. Biol Invasions. 2009; 11: 801–812. https://doi.org/10.1007/s10530-008-9294-2 5. Devereux R, Yates DF, Aukamp J, Quarles RL, Jordan SJ, Stanley RS, et al. Interactions of Thalassia

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testudinum and sediment biogeochemistry in Santa Rosa Sound, NW Florida. Mar Biol Res. 2011; 7: 317–331. https://doi.org/10.1080/17451000.2010.515227 6. Wu ML, Li XY. Research progress on influencing of light attenuation and the associated environmental factors on the growth of submersed aquatic vegetation. Acta Ecol Sin. 2012; 32: 7202–7212. https:// doi.org/10.5846/stxb201110091469 7. Wu J, Cheng SP, Liang W, He F, Wu ZB. Effects of sediment anoxia and light on turion germination and early growth of Potamogeton crispus. Hydrobiologia. 2009; 628: 111–119. https://doi.org/10.1007/ s10750-009-9749-1 8. Phillips GL, Eminson D, Moss B. A mechanism to account for macrophyte decline in progressively eutrophicated freshwaters. Aquat Bot. 1978; 4: 103–126. https://doi.org/10.1016/0304-3770(78) 90012-8 9. Li Y, Zhang JJ, Zhang JQ, Xu WL, Mou ZS. Characteristics of Inorganic Phosphate-Solubilizing Bacte- ria from the Sediments of a Eutrophic Lake. Int J Environ Res Public Health. 2019; 16: 2141. https://doi. org/10.3390/ijerph16122141 PMID: 31212977 10. Srinivasan R, Alagawadi AR, Yandigeri MS, Meena KK, Saxena AK. Characterization of phosphate-sol- ubilizing microorganisms from salt-affected soils of India and their effect on growth of sorghum plants [Sorghum bicolor (L.) Moench]. Ann Microbiol. 2012; 62: 93–105. https://doi.org/10.1007/s13213-011- 0233-6 11. Wu ZH, Sun HM, Yang MY, Lu DX, Yue ST, Fu L. Isolation and identification of phosphate solubilizing bacteria from rice rhizosphere soil, and promoting effect on rice plant growth. J South China Agric Univ.

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2017; 38: 50–57. https://doi.org/10.7671/j.issn.1001-411X.2017.01.009 12. Sun S, Huang X, Fan NJ, Feng ZZ, Li HH, Li SP. Studies on Phosphorus Solubilizing Activity of a Strain Isolated from Crop Rhizosphere. Soils. 2010; 42: 117–122. https://doi.org/10.13758/j.cnki.tr.2010.01. 004 PLOS ONE Screening and biodiversity analysis of cultivable inorganic phosphate–solubilizing bacteria PLOS ONE | https://doi.org/10.1371/journal.pone.0297047 January 19, 2024 10 / 11