Cryptic biodiversity of freshwater fish species in Bangladesh

PLOS One | https://doi.org/10.1371/journal.pone.0318982 April 28, 2025 16 / 18 PLOS One Cryptic biodiversity of freshwater fish seven species that were found in this study but haven’t been named yet, more comprehensive sampling that combines morphological and DNA data would need to be done. Researchers and policymakers can utilize the results of our study, which reveals that Bangladesh is home to many cryptic species, as baseline data. Supporting information S1 Fig. Maximum Likelihood (ML) tree of freshwater species of Bangladesh based on nucleotide sequences of COI gene. (TRE) S1 Table. Uncorrected P distance among the analyzed taxa. (CSV) S2 Table. Result of species delimitation analyses. (TXT) Acknowledgments We are grateful to Associate Professor Dr. A. Kurabayashi, Nagahama Institute of Bio-Science and Technology for some technical support to accomplish the molecular work. Author contributions Conceptualization: Mahmudul Hasan. Data curation: Chiaki Kambayashi. Formal analysis: Chiaki Kambayashi. Funding acquisition: Mahmudul Hasan, Md. Saiful Islam. Investigation: Mahmudul Hasan. Methodology: Mahmudul Hasan, Zahid Hasan Anik. Supervision: Mahmudul Hasan. Writing – original draft: Mahmudul Hasan, Chiaki Kambayashi. Writing – review & editing: Mahmudul Hasan, Chiaki Kambayashi. References 1. Nishat A, Huq SMI, Barua SP, Reza AHMA, Khan ASM. Bio-Ecological Zones of Bangladesh. IUCN, The World Conservation Union, Bangladesh Country Office; 2002. 2. Hasan M, Islam MM, Khan MR, Alam MS, Kurabayashi A, Igawa T, et al. Cryptic anuran biodiversity

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