The climate impact and land use of cultivated meat: Evaluating agricultural feedstock production

26. Burton RJF. The potential impact of synthetic animal protein on livestock production: The new “war against agriculture”? J Rural Stud. 2019; 68:33–45. https://doi.org/10.1016/j.jrurstud.2019.03.002 27. Malerich M, Bryant C. Nomenclature of cell-cultivated meat & seafood products. NPJ Sci Food. 2022; 6(1):56. https://doi.org/10.1038/s41538-022-00172-0 PMID: 36496502 28. Ong S, Choudhury D, Naing MW. Cell-based meat: Current ambiguities with nomenclature. Trends Food Sci Technol. 2020; 102:223–31. https://doi.org/10.1016/j.tifs.2020.02.010 29. Ramani S, Ko D, Kim B, Cho C, Kim W, Jo C, et al. Technical requirements for cultured meat produc- tion: A review. J Anim Sci Technol. 2021; 63(4):681–92. https://doi.org/10.5187/jast.2021.e45 PMID: 34447948 30. Treich N. Cultured meat: Promises and challenges. Environ Resour Econ. 2021; 79(1):33–61. https:// doi.org/10.1007/s10640-021-00551-3 PMID: 33758465 31. Zhang C, Guan X, Yu S, Zhou J, Chen J. Production of meat alternatives using live cells, cultures and plant proteins. Curr Opin Food Sci. 2022; 43:43–52. https://doi.org/10.1016/j.cofs.2021.11.002 32. Post MJ, Levenberg S, Kaplan DL, Genovese N, Fu J, Bryant C, et al. Scientific, sustainability and reg- ulatory challenges of cultured meat. Nat Food. 2020; 1(7):403–15. https://doi.org/10.1038/s43016- 020-0112-z 33. Bryant C, Szejda K, Parekh N, Deshpande V, Tse B. A survey of consumer perceptions of plant-based and clean meat in the USA, India, and China. Front Sustain Food Syst. 2019; 3:11. https://doi.org/10. 3389/fsufs.2019.00011

34. Szejda K, Bryant C, Urbanovich T. US and UK consumer adoption of cultivated meat: A segmentation study. Foods. 2021; 10(5):1050. https://doi.org/10.3390/foods10051050 PMID: 34064740 35. Weinrich R, Strack M, Neugebauer F. Consumer acceptance of cultured meat in Germany. Meat Sci. 2020; 162:107924. https://doi.org/10.1016/j.meatsci.2019.107924 PMID: 31732401 36. Bryant C, Barnett J. Consumer acceptance of cultured meat: An updated review (2018–2020). Appl Sci. 2020; 10(15):5201. https://doi.org/10.3390/app10155201 37. Humbird D. Scale-up economics for cultured meat. Biotechnol Bioeng. 2021; 118(8):3239–50. https:// doi.org/10.1002/bit.27848 PMID: 34101164 38. Lynch J, Pierrehumbert R. Climate impacts of cultured meat and beef cattle. Front Sustain Food Syst. 2019; 3:5. https://doi.org/10.3389/fsufs.2019.00005 PMID: 31535087 39. Risner D, Kim Y, Nguyen C, Siegel JB, Spang ES. Environmental impacts of cultured meat: A cradle- to-gate life cycle assessment [Preprint]. Ecology. 2023. https://doi.org/10.1101/2023.04.21.537778 40. Vergeer R, Sinke P, Odegard I. LCA of cultivated meat Future projections for different scenarios. 2021. CEDelft. 41. Sinke P, Swartz E, Sanctorum H, Van Der Giesen C, Odegard I. Ex-ante life cycle assessment of com- mercial-scale cultivated meat production in 2030. Int J Life Cycle Assess. 2023; 28(3):234–54. https:// doi.org/10.1007/s11367-022-02128-8 42. Tuomisto HL, Allan SJ, Ellis MJ. Prospective life cycle assessment of a bioprocess design for cultured

meat production in hollow fiber bioreactors. Sci Total Environ. 2022; 851:158051. https://doi.org/10. 1016/j.scitotenv.2022.158051 PMID: 35985596 43. Bhat ZF, Morton JD, Mason SL, Bekhit AEA, Bhat HF. Technological, regulatory, and ethical aspects of in vitro meat: A future slaughter-free harvest. Compr Rev Food Sci Food Saf. 2019; 18(4):1192– 208. https://doi.org/10.1111/1541-4337.12473 PMID: 33336995 44. Reis GG, Heidemann MS, Borini FM, Molento CFM. Livestock value chain in transition: Cultivated (cell-based) meat and the need for breakthrough capabilities. Technol Soc. 2020; 62:101286. https:// doi.org/10.1016/j.techsoc.2020.101286 45. Newton P, Blaustein-Rejto D. Social and economic opportunities and challenges of plant-based and cultured meat for rural producers in the US. Front Sustain Food Syst. 2021; 5:624270. https://doi.org/ 10.3389/fsufs.2021.624270 46. O’Neill EN, Cosenza ZA, Baar K, Block DE. Considerations for the development of cost-effective cell culture media for cultivated meat production. Compr Rev Food Sci Food Saf. 2021; 20(1):686–709. https://doi.org/10.1111/1541-4337.12678 PMID: 33325139 47. Kolkmann AM, Post MJ, Rutjens MAM, Van Essen ALM, Moutsatsou P. Serum-free media for the growth of primary bovine myoblasts. Cytotechnology. 2020; 72(1):111–20. https://doi.org/10.1007/ s10616-019-00361-y PMID: 31884572 48. Guan X, Lei Q, Yan Q, Li X, Zhou J, Du G, et al. Trends and ideas in technology, regulation and public acceptance of cultured meat. Future Foods. 2021; 3:100032. https://doi.org/10.1016/j.fufo.2021. 100032 PLOS ONE

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