Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

Results Class Amphibia Linnaeus, 1758 Order Anura Fischer von Waldheim, 1813 Family Bufonidae Gray, 1825 Genus Atelopus Dume´ril and Bibron, 1841 Atelopus podocarpus Coloma, Duellman, Almenda´riz, Ron, Tera´n-Valdez, and Guayasa- min, 2010 Common English name. Podocarpus Harlequin Frog Common Spanish name. Jambato de Podocarpus Etymology. The specific name is a noun in apposition and refers to the Podocarpus National Park. Holotype. QCAZ 6801, adult female, from Lagunas del Compadre (ca. 3300 m), Parque Nacional Podocarpus, Provincia Loja, Ecuador, collected on 1 December 1994 by Jenny Rudston. Paratypes. EPN 1223–26, from Lagunas del Compadre (3400 m), Parque Nacional Podo- carpus, Provincia Loja, Ecuador, no collection date stated on field notes, but possibly collected on September 1981, by Luis H. Albuja V.; EPN 2143–45, from Cerro Toledo (2900 m), Provin- cia Loja, Ecuador, collected on 11 September 1981 by Luis H. Albuja V.; EPN 3235, 3237, Mir- ador path nearby the Centro Administrativo Cajanuma of the Parque Nacional Podocarpus (2850–2990 m), Provincia Loja, Ecuador, obtained on 16–18 November 1986 by Ana Almen- da´riz; KU 120389, from 15 km east of Loja (2700 m), Provincia Zamora Chinchipe, Ecuador, obtained on 10 June 1968 by John D. Lynch; KU 165004–5, from 13 km east of Loja, Abra de Zamora, Provincia Loja, Ecuador, obtained by William E. Duellman; KU 178418, from Provin- cia Zamora Chinchipe: 13.7 km east of Loja, Provincia Loja, Ecuador; KU 201115–7, from 3.7 km south of Saraguro (2800 m), collected on 08 March 1984 by J. E. Simmons; QCAZ 2838

(cleared and double stained skeleton), from Centro Administrativo Cajanuma (2800 m), Par- que Nacional Podocarpus, obtained on 25 June 1987 by Luis A. Coloma, Mario Garcı´a Saltos, and Renato Leo´n. Remarks. Despite numerous searching efforts carried out by different teams from various institutions (including us), in the last several years, in Abra de Zamora and nearby areas, the species was not re-encountered. The last sighting of Atelopus podocarpus dates from 1994, from Parque Nacional Podocarpus (Lagunas del Compadre), and the species is considered probably extinct. Family Hemiphractidae Peters, 1862 Genus Gastrotheca Fitzinger, 1843 Gastrotheca psychrophila Duellman, 1974 Gastrotheca (Duellmania) psychrophila: Dubois, 1987 Gastrotheca (Gastrotheca) psychrophila: Duellman, 2015 Common English name. Ridge Marsupial Frog Common Spanish name. Rana marsupial del Abra de Zamora Etymology. The specific name is derived from the Greek psychros, meaning “cold,” and the Greek philos, meaning “having an affinity for”; the name was used in allusion to the cold cli- mate at the type locality [40]. Holotype. KU 120760, an adult female, from the ridge between Loja and Zamora, 2850 m, 13– 14 km E (by road) of Loja, Provincia Loja, Ecuador; obtained on 10 June 1968 by John D. Lynch. Paratypes. Collected at the type locality: KU 120761, 10 June 1968, John D. Lynch; KU 141586, 21 May 1971, Richard R. Montanucci; KU 142631–7, 21–23 July 1971, William E. Duellman. PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora

PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 8 / 59