Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

33. Zwickl DJ. Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. Ph.D. Thesis, University of Texas, Austin, United States of America; 2006. 34. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985; 39: 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x PMID: 28561359 35. Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioin- formatics. 2003; 19: 1572–1574. https://doi.org/10.1093/bioinformatics/btg180 PMID: 12912839 36. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Anal- ysis Version 6.0. Mol Biol Evol. 2013; 30: 2725–2729. https://doi.org/10.1093/molbev/mst197. PMID: 24132122 37. Cocroft RB, Ryan MJ. Patterns of advertisement call evolution in toads and chorus frogs. Anim Behav. 1995; 49: 283–303. 38. Toledo LF, Martins IA, Bruschi DP, Passos MA, Alexandre C, Haddad CFB. The anuran calling reper- toire in the light of social context. Acta Ethol. 2015; 18: 87–99. 39. Ko¨ hler J, Jansen M, Rodrı´guez A, Kok PJR, Toledo LF, Emmrich M, et al. The use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice. Zootaxa. 2017; 4251: 1–124. https://doi.org/10.11646/zootaxa.4251.1.1 PMID: 28609991 40. Duellman WE. Marsupial Frogs: Gastrotheca and allied genera. Baltimore, MD: JHU Press; 2015. 41. Hedges SB, Duellman WE, Heinicke MP. New World direct-developing frogs (Anura: Terrarana):

molecular phylogeny, classification, biogeography, and conservation. Zootaxa. 2008; 2008: 1–182. 42. Padial JM, Grant T, Frost DR. Molecular systematics of terraranas (Anura: Brachycephaloidea) with an assessment of the effects of alignment and optimality criteria. Zootaxa. 2014; 3825: 1–132. https://doi. org/10.11646/zootaxa.3825.1.1 PMID: 24989881 43. Fouquet A, Gilles A, Vences M, Marty C, Blanc M, Gemmell NJ. Underestimation of species richness in neotropical frogs revealed by mtDNA analyses. PloS One. 2007; 2: e1109. https://doi.org/10.1371/ journal.pone.0001109 PMID: 17971872 44. Keating PL. Effects of anthropogenic disturbances on paramo vegetation in Podocarpus National Park, Ecuador. Phys Geogr. 1998; 19: 221–238. 45. Lynch JD. A review of the Andean Leptodactylid frog genus Phrynopus. Occas Pap Mus Nat Hist. 1975; 35: 1–51. 46. Lynchius flavomaculatus. The IUCN Red List of Threatened Species: e.T57209A89211040. https://dx. doi.org/10.2305/IUCN.UK.2018-1.RLTS.T57209A89211040.en, accessed on 19 May 2020. 2018. 47. IUCN. The IUCN Red List of Threatened Species. Categories and Criteria. Ver. 3.1. http://www. iucnredlist.org/static/categories_criteria_3_1. 2001. 48. Sze´ kely P, Eguiguren JS, Sze´kely D, Ordo´ñez-Delgado L, Armijos-Ojeda D, Riofrı´o-Guama´ n ML, et al. A new minute Pristimantis (Amphibia: Anura: Strabomantidae) from the Andes of southern Ecuador. PloS One. 2018; 13: e0202332. https://doi.org/10.1371/journal.pone.0202332 PMID: 30157209 49. Pristimantis vidua. The IUCN Red List of Threatened Species 2004: e.T57042A11573420. 2004.

50. Pristimantis colodactylus. The IUCN Red List of Threatened Species: e.T56517A89206101. https://dx. doi.org/10.2305/IUCN.UK.2018-1.RLTS.T56517A89206101.en, accessed on 19 May 2020. 2018. 51. Pristimantis cryptomelas. The IUCN Red List of Threatened Species: e.T56539A89206513. https://dx. doi.org/10.2305/IUCN.UK.2019-1.RLTS.T56539A89206513.en. 2019. 52. Aguirre-Mendoza L, Veintimilla D, Salinas K, Aguirre N. Diversidad de anfibios en los pa´ ramos del Par- que Nacional Podocarpus. In: Aguirre N, Ojeda Luna T, Eguiguren P, Aguirre Mendoza Z, editors. Cam- bio clima´tico y biodiversidad: Estudio de caso de los pa´ ramos del Parque Nacional Podocarpus, Ecuador. Loja, Ecuador: Universidad Nacional de Loja, Programa de Biodiversidad y Servicios Ecosis- te´ micos; 2015. 53. Pristimantis percultus. The IUCN Red List of Threatened Species: e.T56845A11544196 https://dx.doi. org/10.2305/IUCN.UK.2004.RLTS.T56845A11544196.en, accessed on 19 May 2020. 2004. 54. Pristimantis balionotus. The IUCN Red List of Threatened Species: e.T56450A11480043 https://dx.doi. org/10.2305/IUCN.UK.2004.RLTS.T56450A11480043.en, accessed on 19 May 2020. 2004. 55. Pristimantis versicolor. The IUCN Red List of Threatened Species: e.T57039A89209992 https://dx.doi. org/10.2305/IUCN.UK.2018-1.RLTS.T57039A89209992.en, accessed on 19 May 2020. 2018. 56. Merino-Viteri A, Coloma LA, Almenda´riz A. Los Telmatobius (Leptodactylidae) de los andes del Ecua- dor y su declive poblacional. In: Lavilla EO, de La Riva I, editors. Estudios sobre las ranas andinas de

los ge´ neros Telmatobius y Batrachophrynus (Anura: Leptodactylidae). Valencia, España: Asociacio´n Herpetolo´gica Española, Monografı´as de Herpetologı´a; 2005. PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 58 / 59