Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

results for samples collected from Abra de Zamora, (Urgiles et al., pers. com.). However, it is possible that the decline of several species in Abra de Zamora could be caused by the chytrid fungus [62]. To assure an adequate protection of the areas that currently are not included in Parque Nacional Podocarpus, we are working on a proposal, in collaboration with the local govern- ments and Ecuadorian Ministry of Environment. Additionally, by the end of 2020, the Action Plan for the conservation of the amphibians of Abra de Zamora will be available, generated by the EcoSs Lab group of Universidad Te´cnica Particular de Loja and supported by funding from the Critical Ecosystem Partnership Fund (CEPF). Supporting information S1 Appendix. Additional specimens examined. (DOCX) S1 Table. Primers used for PCR amplification of 12S rRNA, 16S rRNA, and RAG1 nDNA. (DOCX) S2 Table. Voucher, GenBank accession numbers and locality for the specimens used in the phylogenetic analysis. (DOCX) S3 Table. Uncorrected pairwise distances (%), for the mitochondrial gene 16S fragment, for Lynchius, Pristimantis orestes group and Huicundomantis subgenus of Pristimantis. (XLSX) S4 Table. Information regarding the call recordings and the bioacoustic measurements for each of the recorded males. Values are given as average ± SD (range) and n = sample size. (XLSX) S5 Table. Amphibian species recorded in Abra de Zamora. (XLSX) Acknowledgments We want to thank Enrique Armijos Cano as well all the other rangers of Podocarpus National

Park and the rangers of Reserva Cerro Plateado for their support during our fieldwork. We are grateful for the help and support offered by Ministerio del Ambiente y Agua de Ecuador, Zonal Loja and Zonal Zamora Chinchipe. Special thanks to our former and current students to their valuable help during fieldwork: Santiago Padilla Bustos, Gabriela Mora Castillo, Daniela Sa´nchez Ludeña, Marek Castel Tapia, Stalin Go´mez Eras, Alejandro Ochoa Carrillo, Michelle Angamarca Guartan, Santiago Hualpa Vega, Daniel Hualpa Vega, Maria Cordova Diaz, and Guillermo Enriquez Prado. In addition, we thank Naturaleza y Cultura Internacio- nal, especially Felipe Serrano, and Marı´a F. Ta´pia, Marı´a F. Burneo, Vı´ctor Romero for their support in the implementation of the project. We are grateful to the Tapia Castro family, and especially to Hugo, the administrator of Reserva Madrigal del Podocarpus, for their dedication to the conservation of this amazing place and for granting us access and helping with the field- work. We also want to thank Jose´ Napoleo´n Carrio´n Gordillo for his generous help during our fieldwork in La Siberia hacienda. Research permit was issued by Ministerio del Ambiente del Ecuador (MAE-DNB-CM-2015-0016, granted to Universidad Te´cnica Particular de Loja). PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 55 / 59