Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

present, are shorter, thinner and less defined than in G. lojana [58]. However, the unique advertisement call of G. elicioi (the long, pulsed notes are produced after the short ones, whereas the other Southern Ecuadorian Gastrotheca species tend to produce the long, pulsed notes before the short ones) make it very easy to detect and identify. Remarks. This is a common species in Abra de Zamora and nearby Loja city. Its distribu- tion is restricted to the western slope, close to the city, in evergreen upper montane forest bellow 2600 m. The individuals are usually difficult to encounter, as they are very well camou- flaged in the vegetation; however, the males’ characteristic call can be heard frequently, espe- cially on rainy days. Gastrotheca testudinea (Jime´nez de la Espada, 1870) (Fig 29C) Common English name. Espada’s Marsupial Frog Common Spanish name. Rana marsupial de Jimenez de la Espada Identification. For now, it is the only Gastrotheca species recorded on the eastern slope of Abra de Zamora. It is easily distinguished from all the other species present in Abra de Zamora by its large size, robust body, wide head, flat head top, and characteristic advertisement call [8]. Remarks. This is an uncommon species in Abra de Zamora. It can be encountered on the eastern slope in evergreen lower montane forest (Fig 2D) bellow 2200 m, near the El Tambo sector. Individuals are difficult to find, especially in dense vegetation, but the males’ character- istic call can be heard frequently, especially on rainy days. Family Strabomantidae Pristimantis atratus (Lynch, 1979)

(Fig 29D) Common English name. Santiago Robber Frog Common Spanish name. Cutı´n del Suro Rancho Identification. This small sized species is easily distinguished from all the other Pristiman- tis species present in Abra de Zamora by its whitish, yellowish coloration with pale brown strips, yellowish orange iris, skin on dorsum with low ridges, and black and white (sometimes yellow or orange) markings in axilla, groin and concealed limb surfaces [9]. Fig 29. Other amphibian species present in Abra de Zamora. A. Rhinella margaritifera, from Parque Nacional Podocarpus (Bombuscaro entrance); B. Gastrotheca elicioi, from Loja city; C. Gastrotheca testudínea, from Abra de Zamora; D. Pristimantis atratus, from Abra de Zamora; E. Pristimantis galdi, from Reserva Tapichalaca; F. Pristimantis lymani, from Loja city. https://doi.org/10.1371/journal.pone.0238306.g029 PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 52 / 59