Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

The populations from Reserva Cerro Plateado and Reserva Tapichalaca need a careful revi- sion because the genetic distance between specimens collected in these reserves and the ones from the type locality and nearby areas (northern populations) is around 2.0%. Family Telmatobiidae Fitzinger, 1843 Genus Telmatobius Wiegmann, 1834 Telmatobius cirrhacelis Trueb, 1979 Common English name. Loja Water Frog Common Spanish name. Uco de Loja Etymology. The specific name is derived from the Greek kirrhos, meaning “orange-colored, yellow, tawny”, and kelis, meaning “spot”, with reference to the bright orange spots that char- acterize this species. Holotype. KU 165991, an adult female from 13 km E Loja (Abra de Zamora), 2850 m, Pro- vincia Loja, Ecuador (latitude 03˚59’S, longitude 79˚08’W), taken on 8 March 1975 by William E. Duellman. Paratypes. KU 165989 (male skeleton) and KU 165990 (subadult female) from 15 km E Loja, 2700 m, Provincia Zamora-Chinchipe, Ecuador (latitude 03˚59’S, longitude 79˚07’W). Remarks. Despite numerous searching efforts carried out in the last 40 years [56] the spe- cies was not re-encountered. The last sighting of Telmatobius cirrhacelis dates from 1984, from Parque Nacional Podocarpus (Lagunas del Compadre), and the species is considered probably extinct. Other amphibian species present in Abra de Zamora Besides the 11 species (13, with the two new species presented herein) described from Abra de Zamora, 16 other amphibian species were recorded (S5 Table). Family Bufonidae Rhinella margaritifera (Laurenti, 1768) (Fig 29A)

Common English name. South American Common Toad Common Spanish name. Sapo comu´n sudamericano Identification. It is easily distinguished from all other species present in Abra de Zamora by the presence of developed canthal, antorbital, and supra-orbital crests (larger in females), small, triangular parotoid glands, and granulose skin on dorsum with many small tubercles [57]. Remarks. This is an uncommon species in Abra de Zamora. Can be encountered on the eastern slope, in evergreen lower montane forest (Fig 2D), bellow 2000 m, near the El Tambo sector. Its precise identity has yet to be confirmed, as it is part of a species complex. Family Hemiphractidae Gastrotheca elicioi Carvajal-Endara, Coloma, Morales-Mite, Guayasamin, Sze´kely, and Duellman, 2019 (Fig 29B) Common English name. Elicio’s Marsupial Frog Common Spanish name. Rana marsupial de Elicio Identification. For now, it is the only Gastrotheca species identified on the western slope of Abra de Zamora. Despite our intensive searches, we did not find G. lojana, the other species reported from the area. Based on morphological characters, G. elicioi can be difficult to distin- guish from G. lojana, the main differences being the color pattern (characters of G. lojana in parenthesis): distinct dark canthal stripes present (absent), groin and anterior and posterior surfaces of the thighs slightly mottled (heavily mottled), and the dark bars on the limbs, when PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 51 / 59