Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

are composed usually by one or two notes (frequency modulated tonal sounds), but sometimes (probably in case of social interactions, triggered by the nearby presence of a female or a com- petitive male) the males can emit calls with up to 8 notes (FUTPL-A 230, Fig 26E and 26f). The calls are characterized by a mean duration of 0.069 s (SD = 0.005), a mean inter-call interval of 6.075 s (SD = 2.371) and a mean call rate of 10.0 calls/min (SD = 1.610). In the case of the dou- ble-noted calls the call duration was on average 0.355 s (SD = 0.110) and had a mean note rate Fig 26. Advertisement call of Pristimantis balionotus. A–D MUTPL 677, FUTPL-A 232: A. Oscillogram of a 6 calls section of the call series, with single and double-noted calls; B. Oscillogram of a single-noted call; C. Spectrogram of a single-noted call; D. Power spectrum of a single-noted call; E–F FUTPL-A 230: E. Oscillogram of a 6 notes section of a 8-noted call; F. Spectrogram of a 6 notes section of a 8-noted call. https://doi.org/10.1371/journal.pone.0238306.g026 PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 46 / 59