Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

Pristimantis percultus (Lynch, 1979) (Fig 24) Eleutherodactylus percultus Lynch, 1979 Eleutherodactylus (Eleutherodactylus) percultus: Lynch, 1996; Lynch and Duellman, 1997 Pristimantis percultus: Heinicke, Duellman, and Hedges, 2007 Table 3. Body mass (in grams), measurements (in mm) and morphological proportions (in percentages) of adult females and males of the species from the Subge- nus Huicundomantis described from Abra de Zamora. Character Pristimantis cryptomelas Pristimantis percultus Pristimantis balionotus Pristimantis versicolor females (n = 3) males (n = 2) females� (n = 3) males� (n = 1) females� (n = 2) males (n = 6) females (n = 3) males (n = 4) Body mass (BM) 3.39 ± 2.69 (1.68– 6.49) 1.19–1.84 2.08–2.52 - 2.48 1.12 ± 0.21 (0.88– 1.44) 2.13 ± 0.69 (1.62– 2.92) 1.05 ± 0.13 (0.96– 1.24) Snout-vent length (SVL) 35.3 ± 9.06 (28.9– 45.7) 25.6–31.2 33.6 ± 4.15 (30.1– 38.2) 29.8 28.4–32.4 24.7 ± 0.83 (23.6– 25.8) 30.8 ± 1.40 (29.4– 32.2) 24.0 ± 1.08 (22.9– 25.4) Head width (HW) 12.5 ± 3.38 (10.5– 16.4) 9.6–11.6 13.1 ± 1.71 (11.7– 15.0) 12.1 11.3–11.5 9.0 ± 0.47 (8.4– 9.8) 11.5 ± 1.26 (10.3– 12.8) 8.8 ± 0.39 (8.4– 9.2) Head length (HL) 11.6 ± 3.15 (9.7– 15.2) 7.6–9.5 11.9 ± 1.30 (10.6– 13.2) 11.0 10.4–10.7 8.7 ± 0.50 (7.9– 9.3) 10.3 ± 0.95 (9.4– 11.3) 8.0 ± 0.84 (7.2– 8.9) Interorbital distance (IOD) 4.0 ± 1.00 (3.2– 5.1) 2.8–3.4 4.0 ± 0.30 (3.7– 4.3) 3.9 2.8–4.0 3.0 ± 0.49 (2.6– 3.9) 3.3 ± 0.27 (3.1– 3.6) 2.5 ± 0.19 (2.4– 2.8) Internarial distance (IND) 2.7 ± 0.46 (2.4– 3.2) 2.1–2.5 2.4–2.6 - 2.4 2.0 ± 0.09 (1.9– 2.1) 2.4 ± 0.17 (2.2–

2.5) 1.9 ± 0.08 (1.8– 2.0) Upper eyelid width (EW) 3.3 ± 0.78 (2.7– 4.2) 2.3–2.9 3.0 ± 0.21 (2.8– 3.2) 3.4 2.6–3.1 2.5 ± 0.09 (2.4– 2.6) 2.8 ± 0.10 (2.7– 2.9) 2.5 ± 0.06 (2.4– 2.5) Eye diameter (ED) 4.0 ± 1.19 (3.3– 5.4) 3.0–3.6 3.8 ± 0.55 (3.4– 4.4) 3.5 3.3 2.9 ± 0.14 (2.8– 3.1) 3.5 ± 0.15 (3.3– 3.6) 2.9 ± 0.06 (2.8– 2.9) Eye-nostril distance (EN) 3.6 ± 1.29 (2.5– 5.0) 2.4–3.5 3.6 ± 0.58 (3.3– 4.3) 3.4 3.6–4.0 2.7 ± 0.15 (2.5– 2.9) 3.1 ± 0.15 (3.0– 3.3) 2.6 ± 0.22 (2.3– 2.8) Tympanum diameter (TD) 1.1 ± 0.21 (0.9– 1.3) 0.9–1.1 1.8 ± 0.20 (1.6– 2.0) 1.5 1.7–1.8 1.4 ± 0.11 (1.3– 1.6) 1.6 ± 0.15 (1.4– 1.7) 1.3 ± 0.08 (1.2– 1.4) Femur length (FL) 16.1 ± 4.16 (13.6– 20.9) 12.3–14.7 15.2–17.3 - 13.3 10.6 ± 0.30 (10.2– 10.8) 14.5 ± 1.05 (13.5– 15.6) 11.0 ± 0.44 (10.4– 11.4) Tibia length (TL) 18.3 ± 3.92 (15.5– 22.8) 14.4–15.2 18.6 ± 2.33 (16.7– 21.2) 15.5 12.8–14.3 11.5 ± 0.24 (11.2– 11.9) 16.4 ± 0.72 (15.8– 17.2) 12.6 ± 0.72 (11.8– 13.5) Foot length (FoL) 17.4 ± 4.77 (14.1– 22.9) 12.4–15.3 16.0–16.6 - 13.1 9.8 ± 0.30 (9.3– 10.2) 14.6 ± 1.23 (13.7– 16.0) 11.4 ± 0.81 (10.4– 12.3) Hand length (HaL) 11.2 ± 3.71 (8.5– 15.4) 8.2–9.3 9.6–10.8 - 7.7 6.4 ± 0.33 (6.0– 6.9) 9.8 ± 1.15 (8.9– 11.1) 7.3 ± 0.43 (6.9– 7.9) HW/SVL 33.8–36.3 37.2–37.5 38.7–39.3 40.6 34.9–40.5 34.1–38.1 35.0–39.8 36.2–37.0 HL/SVL 31.2–33.6 29.7–30.4 34.6–36.8 36.9 32.1–37.7 31.7–36.8 32.0–35.1 31.1–35.0 HL/HW 92.4–92.7 79.2–81.9 88.0–95.2 90.9 92.0–93.0 88.8–98.9 88.3–91.3 85.9–96.7

EN/HL 25.8–32.9 31.6–36.8 27.5–32.6 30.9 33.6–38.5 30.1–34.2 29.2–31.9 31.5–34.2 ED/HL 33.7–35.5 37.9–39.5 29.2–33.3 31.8 30.8–31.7 31.1–36.7 31.9–35.1 32.6–38.9 EW/IOD 82.4–86.1 82.1–85.3 72.5–75.7 87.2 65.0–110.7 66.7–96.2 80.6–87.5 89.3–104.2 EN/ED 73.5–97.0 80.0–97.2 94.3–97.7 97.1 109.1–121.2 87.1–103.6 88.6–91.7 82.1–96.6 TD/ED 22.2–39.4 30.0–30.6 45.5–51.4 42.9 51.5–54.5 44.8–51.6 42.4–47.2 42.9–48.3 FL/SVL 43.9–47.1 47.1–48.0 50.5–53.1 - 41.0 41.9–43.9 45.9–48.4 44.3–47.2 TL/SVL 49.9–53.6 48.7–56.3 54.9–55.5 52.0 44.1–45.1 44.6–49.8 52.4–53.7 50.2–53.7 FoL/SVL 48.7–50.1 48.4–49.0 50.9–53.2 - 40.4 38.4–42.7 45.6–49.7 45.4–48.4 HaL/SVL 29.4–33.7 29.8–32.0 31.9–33.1 - 23.8 24.1–26.7 30.3–34.5 30.0–31.1 Values are given as mean ± SD (range). Female body mass includes eggs. �—indicates that some measurements were included from the original descriptions [9]. https://doi.org/10.1371/journal.pone.0238306.t003 PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 41 / 59