Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

Diagnosis. Pristimantis cryptomelas is a medium sized species distinguished by the follow- ing combination of traits: (1) skin on dorsum shagreened; skin on venter areolate; discoidal fold weak; dorsolateral folds absent; low middorsal fold present; (2) tympanic membrane absent but tympanic annulus evident, oval, its length about 30% of the length of eye; Fig 21. Maximum likelihood phylogram of the Huicundomantis subgenus of Pristimantis based on 2463 base pairs of concatenated DNA from 12S, 16S, and RAG-1 gene fragments. Bootstrap values (%) and Bayesian posterior probabilities (decimal) are shown except when they are below 50 (bootstrap) or 0.5 (posterior probability); asterisks indicate support values of 100% or 1 (posterior probabilities). Outgroup is not shown; the tree was routed with Pristimantis galdi. With green are marked the newly generated sequences for the present study. The collection number, species name, province and short locality name of the samples are shown next to each terminal; all samples are from Ecuador (associated data listed in S2 Table). Abbreviations: CCS = confirmed candidate species, UCS = unconfirmed candidate species [30]. https://doi.org/10.1371/journal.pone.0238306.g021 PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 38 / 59