Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

national election. A tireless fighter for women’s rights, she laid important foundations for the development of women in academia and science, nationally and in South America. In Ecua- dor, there are currently many women involved in biological sciences and with this species, in addition to being a tribute to Matilde, we honor their tireless work for the conservation of bio- logical diversity. Holotype. MUTPL 731 (Figs 16–18), field no. SC 958, an adult male from Ecuador, Loja Province, Abra de Zamora (3.9910˚ S, 79.1458˚ W; datum WGS84), 2815 m above sea level, collected by Paul Sze´kely, Stalin Go´mez and Alejandro Ochoa on 6 August 2019. Paratypes (4 females, 2 males and 1 juvenile). Three specimens collected in the type locality: MUTPL 733 (SC 960), a juvenile (Fig 19F) collected syntopically with the holotype; MUTPL 394 (SC 152), an adult female (Fig 19B) collected by Diana Sze´kely, Veronica Urgiles, Anna Savage and Paul Sze´kely (3.9910˚ S, 79.1455˚ W; datum WGS84), 2815 m, on 31 May 2018; MUTPL 366 (SC 179), an adult female collected by Paul Sze´kely (3.9912˚ S, 79.1460˚ W; datum WGS84), 2817 m, on 19 July 2018; four specimens from Ecuador, Loja Province, Caja- numa sector, Parque Nacional Podocarpus: MUTPL 361 (SC 174) an adult female (Fig 19A) and MUTPL 360 (SC 173) and MUTPL 362 (SC 175) adult males (Fig 19D and 19E) collected by Diana Sze´kely and Paul Sze´kely (4.1141˚ S, 79.1627˚ W; datum WGS84), 3361 m, on 29 June 2018; MUTPL 813 (SC 1113), an adult female (Fig 19C) collected by Diana Sze´kely and

Paul Sze´kely (4.1054˚ S, 79.1616˚ W; datum WGS84), 3347 m, on 23 November 2019. Diagnosis. We assign this species to Pristimantis based on phylogenetic evidence (Fig 7) and on the general morphological similarity to other members of the genus. Pristimantis matil- dae is a small species distinguished by the following combination of traits: (1) skin on dorsum finely tuberculated (trait more visible in life); skin on venter areolate; discoidal fold and dorso- lateral folds absent; low middorsal fold present; (2) tympanic annulus and tympanic mem- brane absent; supratympanic fold absent; (3) snout moderately long, subacuminate in dorsal view and subacuminate and slightly protruding in profile; canthus rostralis weakly concave in dorsal view, angular in profile; (4) upper eyelid bearing one prominent tubercle and several small tubercles, about 77% IOD in females and 79% IOD in males; cranial crests absent; (5) dentigerous processes of vomers concealed in buccal mucosa; each processes bearing 2 to 5 Fig 19. Color variation of Pristimantis matildae sp. nov. in life. A–C females, paratypes: A. MUTPL 361, B. MUTPL 394, C. MUTPL 813; D–E males, paratypes: D. MUTPL 362, E. MUTPL 360; F. juvenile, paratype: MUTPL 733. Abra de Zamora (B, F), Parque Nacional Podocarpus—Cajanuma (A, C, D, E). https://doi.org/10.1371/journal.pone.0238306.g019 PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 33 / 59