Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

IOD in males; cranial crests absent; (5) dentigerous processes of vomers prominent, oblique, triangular or oval in outline, separated medially by distance almost equal with the width of processes; each processes bearing 4 to 6 teeth; (6) males with large subgular vocal sac and vocal slits; nuptial pads absent; (7) Finger I shorter than Finger II; discs on fingers just slightly expanded, rounded; circumferential grooves present; fingers lacking lateral fringes; (8) subarti- cular tubercles prominent, round and subconical in section; supernumerary palmar tubercles rounded, large, just slightly smaller than subarticular tubercles; palmar tubercle completely divided into a larger (inner) and a smaller (outer) tubercles; thenar tubercle oval, smaller than the inner palmar tubercle; (9) ulnar tubercles absent or very small, inconspicuous (trait more visible in life); (10) heel with several small tubercles (trait more visible in life); outer edge of tarsus with a row of small tubercles (trait more visible in life); inner tarsal tubercles coalesced into a long tarsal fold; (11) inner metatarsal tubercle broadly ovoid, about 3x elongated and subconical outer metatarsal tubercle; supernumerary plantar tubercles ovoid, large, just slightly smaller than subarticular tubercles; (12) Toe V slightly longer than Toe III; discs on toes just slightly expanded, rounded, about same size as those on fingers; circumferential grooves pres- ent; toes lacking lateral fringes; webbing basal; (13) evident sexual dimorphism: in life, the

females with dorsum, flanks, dorsal surfaces of hindlimbs and arms dark brown with various white, irregular spots and dark gray venter; males dorsum light brown, reddish brown or red, without white spots and much lighter venter, pinkish white or light gray; no markings in axilla, groin or on concealed limb surfaces; iris whitish gray with a reddish bronze, broad, median Fig 11. Holotype of Pristimantis samaniegoi sp. nov. (MUTPL 357, adult female) in preservative. A. Dorsal view; B. Ventral view; C. Lateral view. https://doi.org/10.1371/journal.pone.0238306.g011 PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 23 / 59