Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

Ochoa, Michelle Angamarca and Paul Sze´kely (3.9836˚ S, 79.1307˚ W; datum WGS84), 2569 m, on 27 April 2019; MUTPL 674 (SC 993), an adult male (Fig 13E) collected by Paul Sze´kely, Stalin Go´mez, Alejandro Ochoa and Michelle Angamarca (3.9928˚ S, 79.1469˚ W; datum WGS84), 2865 m, on 17 May 2019; MUTPL 676 (SC 936), an adult female (Fig 13B) collected by Paul Sze´kely, Stalin Go´mez and Alejandro Ochoa (3.9869˚ S, 79.1443˚ W; datum WGS84), 2827 m, on 24 May 2019; MUTPL 777 (SC 1030) an adult female (Fig 13C) collected by Stalin Go´mez and Alejandro Ochoa (3.9965˚ S, 79.1469˚ W; datum WGS84), 2891 m, on 22 Septem- ber 2019. Diagnosis. We assign this species to Pristimantis based on phylogenetic evidence (Fig 7) and on the general morphological similarity to other members of the genus. Pristimantis sama- niegoi is a small species, distinguished by the following combination of traits: (1) skin on dor- sum finely tuberculated (in life the skin tuberculated texture is more evident); skin on venter coarsely areolate; thoracic and discoidal folds evident (trait more visible in preservative); dor- solateral folds absent; low middorsal fold present; (2) tympanic annulus and tympanic mem- brane absent; supratympanic fold present; (3) snout short, rounded in dorsal view and in profile; canthus rostralis weakly concave in dorsal view, rounded in profile; (4) upper eyelid bearing several small tubercles (trait more visible in life), about 67% IOD in females and 70% Fig 10. Holotype of Pristimantis samaniegoi sp. nov. (MUTPL 357, adult female), SVL 20.1 mm, in life. A. Lateral

view; B. Ventral view; C. Dorsal view. https://doi.org/10.1371/journal.pone.0238306.g010 PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 22 / 59