Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

the base of the shrubs. This is a common species. Calling males were encountered all year round but more frequently on rainy nights. Conservation status. Pristimantis vidua is currently categorized as Endangered [49] based on criteria B1 ab(iii). Even if this is a relatively abundant species at all localities, and its habitat does not face any major threats (it is encountered in one national protected area and two pri- vate and/or community protected areas), we suggest maintaining this category. This is due the fact that the species Extent of occurrence (EOO) and Area of occupancy (AOO) are estimated to be less than 200 km2, it is currently known only from 4 localities, and the existing popula- tions are severely fragmented. Remarks. Lynch [9] provides a detailed description of this species, including a brief description of the cranial osteology. Our diagnosis concurs with most of the morphological features described by the author (characters given in parentheses), but we also added descrip- tions of the characters that are more evident in life. Some of the differences that we observed are: skin on dorsum finely tuberculated (in contrast to the finely shagreened dorsum described by Lynch); dentigerous processes of vomers bearing 3 to 9 teeth (2 to 4 teeth); small, inconspic- uous, ulnar tubercles present (no ulnar tubercles or fold); heel with 2 or 3 larger and several smaller tubercles (no heel tubercles); inner tarsal tubercles coalesced into a long tarsal fold (tar- sus bearing ill-defined tubercle along inner edge). The diagnosis provided herein is based on

12 specimens from the original description (KU 120082–89, 120090–92, 141994), 3 adult females (MUTPL 295, 312, 805) and 6 adult males (MUTPL 296, 495–499, 501), collected from the type locality and 25 specimens collected from the other localities. Pristimantis vidua is part of the P. orestes subgroup, and is most closely related to an unde- scribed species from Parque Nacional Podocarpus (Lagunas del Compadre). However, its exact position in the subgroup remains non-resolved (Fig 7). The genetic distance between P. vidua and its closest relatives from the P. orestes subgroup ranges between 4.6–9.2% (S3 Table). Pristimantis samaniegoi sp. nov. Sze´kely, Eguiguren, Ordo´ñez-Delgado, Armijos-Ojeda, and Sze´kely urn:lsid:zoobank.org:act:0B9E2517-1BE9-4361-BB7D-D5B34120C248 (Figs 10–13) Common English name. Samaniego’s Rain Frog Common Spanish name. Cutı´n de Samaniego Etymology. The specific epithet is a noun in the genitive case and is a patronym for Dr. Gustavo Samaniego Rodrı´guez. The name is given as a tribute to his important contribution to the conservation of biodiversity in southern Ecuador. Through a generous donation of an important part of his estate, he contributed to the creation of the administrative and interpre- tation center in the Cajanuma sector of the Parque Nacional Podocarpus, which is one of the most important Ecuadorian sites of endemism and biodiversity. He was a university professor for over 40 years, and currently still maintains the "Reserva El Cristal" agro-ecological farm as an example of sustainable land management.

Holotype. MUTPL 357 (Figs 10–13A), field no. SC 170, an adult female from Ecuador, Loja Province, Cajanuma entrance to the Parque Nacional Podocarpus, on Los Miradores trail (4.1151˚ S, 79.1651˚ W; datum WGS84), 3258 m above sea level, collected by Paul Sze´kely and Diana Sze´kely on 28 June 2018. Paratypes (3 females, 3 males and 1 juvenile). Two specimens collected syntopically with the holotype: MUTPL 356 (SC 169) and MUTPL 358 (SC 171), both adult males (Fig 13A and 13D). Five specimens from Ecuador, Loja Province, Abra de Zamora: MUTPL 365 (SC 178), an adult female collected by Paul Sze´kely (3.9912˚ S, 79.1460˚ W; datum WGS84), 2817 m, on 19 July 2018; MUTPL 660 (SC 253), a juvenile (Fig 13F) collected by Stalin Go´mez, Alejandro PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 21 / 59