Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

encountered individuals proved to be females. We thus agree that the species has a female- biased sex ratio; alternatively, the detectability might be different between the sexes, as usually the females were found perched on leaves (at 20 cm to 1.5 m above the ground), while the males were encountered somewhat hidden in the vegetation or calling from inside the moss at Fig 9. Advertisement call of Pristimantis vidua (FUTPL-A 239). A. Oscillogram of a 10 calls section of the call series; B. Oscillogram of a single call; C. Spectrogram of a single call; D. Power spectrum of a single call. https://doi.org/10.1371/journal.pone.0238306.g009 PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 20 / 59