Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

Advertisement call. The advertisement call of one male from Abra de Zamora was recorded in 2017 and of two males from Reserva Madrigal del Podocarpus in 2018. Descriptive statistics of the acoustic variables are provided in Table 2 (the detailed information of each of the separate recordings is presented in the S4 Table). Pristimantis vidua has an advertisement Table 1. Body mass (in grams), measurements (in mm) and morphological proportions (in percentages) of adult females and males of the species from the Pristi- mantis orestes species group described from Abra de Zamora. Character Pristimantis vidua Pristimantis samaniegoi sp. nov. Pristimantis colodactylus Pristimantis matildae sp. nov. females (n = 16) males (n = 11) females (n = 4) males (n = 3) females (n = 2) males (n = 1) females (n = 4) males (n = 3) Body mass (BM) 0.97 ± 0.29 (0.54– 1.61) 0.49 ± 0.10 (0.35– 0.66) 0.63 ± 0.15 (0.44– 0.80) 0.34 ± 0.04 (0.31– 0.38) 0.61–0.62 0.31 0.84 ± 0.16 (0.66– 0.97) 0.55 ± 0.06 (0.50– 0.61) Snout-vent length (SVL) 22.4 ± 2.35 (17.9– 26.8) 17.6 ± 1.28 (16.1– 19.5) 19.3 ± 1.58 (17.1– 20.7) 16.0 ± 1.17 (14.7– 16.9) 20.2–20.5 16.9 22.5 ± 1.12 (20.8– 23.3) 20.4 ± 0.68 (19.9– 21.2) Head width (HW) 8.2 ± 0.85 (6.9– 10.1) 6.3 ± 0.54 (5.7– 7.2) 6.6 ± 0.37 (6.1– 7.0) 5.3 ± 0.47 (4.8– 5.7) 6.5–6.9 5.4 8.1 ± 0.27 (7.7– 8.3) 6.9 ± 0.35 (6.7– 7.3) Head length (HL) 7.4 ± 0.90 (5.5– 9.2) 5.6 ± 0.53 (4.6– 6.4) 5.9 ± 0.28 (5.6– 6.2) 4.7 ± 0.44 (4.2– 5.0) 5.9–6.0 5.3 7.2 ± 0.15 (7.0– 7.3) 6.5 ± 0.27 (6.2– 6.7) Interorbital distance (IOD) 2.6 ± 0.24 (2.2– 3.1) 2.1 ± 0.13 (1.9–

2.3) 2.4 ± 0.13 (2.2– 2.5) 1.9 ± 0.17 (1.7– 2.0) 2.3–2.4 1.8 2.5 ± 0.24 (2.3– 2.8) 2.2 ± 0.10 (2.1– 2.3) Internarial distance (IND) 2.0 ± 0.17 (1.7– 2.3) 1.7 ± 0.19 (1.4– 2.0) 1.8 ± 0.22 (1.5– 2.0) 1.5 ± 0.15 (1.4– 1.7) 1.6 1.4 1.9 ± 0.10 (1.8– 2.0) 1.9 ± 0.12 (1.8– 2.0) Upper eyelid width (EW) 1.6 ± 0.21 (1.3– 1.9) 1.4 ± 0.18 (1.2– 1.7) 1.6 ± 0.13 (1.4– 1.7) 1.3 ± 0.06 (1.3– 1.4) 1.4–1.5 1.3 1.9 ± 0.14 (1.7– 2.0) 1.7 ± 0.15 (1.6– 1.9) Eye diameter (ED) 2.4 ± 0.27 (2.0– 2.8) 2.0 ± 0.18 (1.8– 2.3) 2.1 ± 0.10 (2.0– 2.2) 1.7 ± 0.15 (1.6– 1.9) 2.1 1.9 2.3 ± 0.08 (2.2– 2.4) 2.2 ± 0.06 (2.1– 2.2) Eye-nostril distance (EN) 1.8 ± 0.26 (1.4– 2.3) 1.3 ± 0.13 (1.1– 1.5) 1.7 ± 0.08 (1.6– 1.8) 1.4 ± 0.06 (1.3– 1.4) 1.8–1.9 1.6 2.1 ± 0.05 (2.0– 2.1) 1.8 ± 0.10 (1.7– 1.9) Tympanum diameter (TD) 1.0 ± 0.19 (0.8– 1.4) 0.9 ± 0.14 (0.6– 1.1) - - - - - - Femur length (FL) 9.0 ± 0.55 (7.4– 10.0) 7.2 ± 0.59 (6.3– 8.0) 7.7 ± 0.42 (7.3– 8.3) 6.3 ± 0.44 (5.8– 6.60) 8.1–8.3 7.0 9.5 ± 0.66 (9.1– 10.5) 8.9 ± 0.36 (8.6– 9.30 Tibia length (TL) 9.5 ± 0.58 (8.4– 10.4) 7.5 ± 0.39 (6.7– 8.2) 8.3 ± 0.35 (7.9– 8.7) 6.7 ± 0.35 (6.4– 7.1) 8.6–9.0 7.6 10.5 ± 0.49 (10.2– 11.2) 9.4 ± 0.56 (8.9– 10.0) Foot length (FoL) 8.9 ± 0.85 (7.6– 10.2) 6.9 ± 0.55 (5.7– 7.5) 7.7 ± 0.45 (7.3– 8.3) 5.9 ± 0.25 (5.7– 6.2) 7.2–7.5 6.1 9.1 ± 0.50 (8.7– 9.8) 8.1 ± 0.38 (7.8– 8.5) Hand length (HaL) 5.6 ± 0.62 (4.4– 6.9) 4.2 ± 0.27 (3.7– 4.7) 4.7 ± 0.39 (4.2– 5.1) 3.6 ± 0.31 (3.3– 3.9) 4.3–4.4 3.7 5.7 ± 0.34 (5.3– 6.1) 5.2 ± 0.12 (5.1– 5.3)

HW/SVL 34.5–38.6 34.6–37.1 32.8–35.7 32.7–33.7 32.2–33.7 32.0 35.6–37.0 33.2–34.4 HL/SVL 26.6–36.9 28.6–33.9 30.0–32.7 28.6–29.7 29.2–29.3 31.4 31.3–33.7 31.2–32.7 HL/HW 76.4–98.6 80.7–96.8 87.9–92.4 87.5–89.1 87.0–90.8 98.1 88.0–90.9 91.8–98.5 EN/HL 22.4–30.3 20.8–26.1 27.4–30.4 28.0–31.0 30.0–32.2 30.2 28.6–28.8 27.3–28.4 ED/HL 30.1–40.0 32.2–40.0 33.9–36.1 34.7–38.1 35.0–35.6 35.8 31.4–32.9 32.8–33.9 EW/IOD 51.7–73.1 60.0–80.0 63.6–70.8 65.0–76.5 60.9–62.5 72.2 71.4–82.6 76.2–82.6 EN/ED 66.7–92.0 57.9–79.0 80.0–85.0 73.7–82.4 85.7–90.5 84.2 87.5–91.3 81.0–86.4 TD/ED 34.8–51.9 33.3–47.8 - - - - - - FL/SVL 35.9–46.0 36.3–46.6 38.3–42.7 38.5–40.0 39.5–41.1 41.4 40.4–45.1 43.2–43.9 TL/SVL 37.6–48.6 38.5–46.6 40.3–49.1 39.6–43.5 42.0–44.6 45.0 44.7–49.0 44.7–47.2 FoL/SVL 35.2–43.8 35.4–42.9 37.8–42.7 35.8–38.8 35.1–37.1 36.1 38.6–42.1 39.1–40.1 HaL/SVL 22.6–27.5 21.2–25.8 21.9–26.3 22.4–23.1 21.0–21.8 21.9 24.0–26.2 25.0–25.6 Values are given as mean ± SD (range). Female body mass includes eggs. https://doi.org/10.1371/journal.pone.0238306.t001 PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 18 / 59