Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

conclusive conclusions are hard to reach due to the species low detectability. The female MUTPL 342 had 31 large unpigmented eggs (SVL 42.5 mm, BM 8.21 g). Conservation status. Lynchius flavomaculatus is currently listed as Data Deficient in view of the absence of recent information on its extent of occurrence, population status and ecologi- cal requirements [46]. Based on the available (revised) information we consider L. flavomacu- latus to be Endangered following B1ab(iv)c(iii)+2ab(iv)c(iii) IUCN criteria [47] because: (1) its Extent of occurrence (EOO) and Area of occupancy (AOO) are estimated to be less than 50 km2; (2) it is known from only two close locations; and (3) we suspect a decline in the number of locations or subpopulations. Remarks. The diagnosis provided herein is based on the thorough revision of Motta et al. [27] and one adult female specimen, MUTPL 342, collected from about 13 km south to the type locality. Our diagnosis concurs with most of the morphological features described; how- ever we identified some differences, which we attribute to the fact that the specimens examined by the authors [27] belong to at least four different cryptic species. Also, the opportunity to examine a live specimen helped to complete the diagnosis by adding descriptions of characters which are evident in life, but lost in preserved specimens. The most important difference is the Fig 6. Distribution of Lynchius flavomaculatus and of the species from the Pristimantis orestes species group described from Abra de Zamora. Records are

based on specimens (and DNA sequences) deposited at the Museo de Zoologı´a, Universidad Te´cnica Particular de Loja (MUTPL). 1. Bosque Protector Washapamba, 2. Reserva Madrigal del Podocarpus, 3. Parque Nacional Podocarpus (Cajanuma sector). https://doi.org/10.1371/journal.pone.0238306.g006 PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 13 / 59