Fifty years after: A taxonomic revision of the amphibian species from the Ecuadorian biodiversity hotspot Abra de Zamora, with description of two new Pristimantis species

on the available data we conclude that the specimen KU 218210 collected at 19.4 km south of Yangana, Loja province, is most likely a different, undescribed species. Also, the specimens collected at 8–9 km north to San Lucas or 10 km south of Saraguro, in Loja province [27], actually belong to a currently undescribed new species. As for the specimens from Morona Santiago province and Peru [25, 27] they represent very probable different, undescribed species, taking in consideration the large distances from the type locality. The species was encountered at an altitudinal range between 3000 and 3250 m a.s.l. in shrub pa´ramo ecosystems. Natural history. This species is considered terrestrial, usually found beneath rocks or logs in pa´ramo and subpa´ramo habitats [45]. We found the female MUTPL 342 emerging from a thick moss bed, which is likely a preferred microhabitat for Lynchius species. From the type locality, there were collected 44 specimens between 1968 and 1984 (22 specimens in 1968, 7 in 1971, 14 in 1975, and 1 in 1984) by J.D. Lynch, R.R. Montanucci and W.E. Duellman, accord- ing to the Kansas University Herpetology Collections database (https://biodiversity.ku.edu/ herpetology/collections). However, our ongoing fieldwork since 2016 in the type locality did not yield any specimens. It is possible that the population is experiencing a decline, but Fig 5. Release call of Lynchius flavomaculatus (MUTPL 342, FUTPL-A 246). A. Oscillogram of a single call; B. Spectrogram of a single call; C. Power spectrum of a single call. https://doi.org/10.1371/journal.pone.0238306.g005

PLOS ONE Taxonomic revision of the amphibian species from Abra de Zamora PLOS ONE | https://doi.org/10.1371/journal.pone.0238306 September 10, 2020 12 / 59